Document Detail


Glycoform quantification of chondroitin/dermatan sulfate using a liquid chromatography-tandem mass spectrometry platform.
MedLine Citation:
PMID:  16475824     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chondroitin sulfate (CS) is a glycosaminoglycan consisting of repeating uronic acid, N-acetylgalactosamine disaccharide units {[HexAbeta/alpha(1-3)GalNAcbeta(1-4)](n)()}. CS chains are polydisperse with respect to chain length, sulfate content, and glucuronic acid epimerization content, resulting in a distribution of glycoforms for a chain bound to any given serine residue. Usually, CS glycoforms exist, differing in sulfation position and uronic acid epimerization. This work introduces a novel LC-MS/MS platform for the quantification of mixtures of CS oligosaccharides. The CS polysaccharides were partially depolymerized and labeled with either the light (d(0)) or heavy (d(4)) form of 2-anthranilic acid (2-AA). Excess reagent was removed, and mixtures of the CS standard (d(0)) and unknown (d(4)) were made. The CS mixture was subjected to size exclusion chromatography (SEC) with on-line electrospray ionization mass spectrometric detection in the negative ion mode. Tandem mass spectra were acquired, and quantification of unknown samples within the mixture was made using relative ion abundances of specific diagnostic ions. The high accuracy and precision of the glycomics platform were demonstrated using glycoform mixtures made from standard CS preparations. The CS glycoform analysis method was then applied to cartilage extract, versican, and several dermatan sulfate preparations. This work presents the first application of a glycomics platform for the quantification of CS oligosaccharide mixtures for obtaining specific information about the positions of GalNAc sulfation and uronic acid epimerization.
Authors:
Alicia M Hitchcock; Catherine E Costello; Joseph Zaia
Related Documents :
12485794 - Influence of different crosslinking treatments on the physical properties of collagen m...
8268214 - Charge isomers of urinary bikunin (trypsin inhibitor).
24753374 - N-acyl amino acids and their impact on biological processes.
24108064 - Cell-free cartilage engineering approach using hyaluronic acid-polycaprolactone scaffol...
9882684 - S-methylmethionine metabolism in escherichia coli.
18808414 - Function of oleic acid on epidermal barrier and calcium influx into keratinocytes is as...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Biochemistry     Volume:  45     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-14     Completed Date:  2006-04-10     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2350-61     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Carbohydrate Sequence
Cartilage / chemistry
Cattle
Chondroitin ABC Lyase / metabolism
Chondroitin Sulfate Proteoglycans / chemistry
Chondroitin Sulfates / analysis*
Chromatography, Liquid / methods*
Dermatan Sulfate / analogs & derivatives*,  analysis
Isotope Labeling
Lectins, C-Type / chemistry
Mass Spectrometry / methods
Molecular Sequence Data
Versicans
Grant Support
ID/Acronym/Agency:
P41 RR010888/RR/NCRR NIH HHS; P41 RR010888-080102/RR/NCRR NIH HHS; P41 RR010888-080142/RR/NCRR NIH HHS; P41 RR010888-095173/RR/NCRR NIH HHS; P41 RR010888-118113/RR/NCRR NIH HHS; P41 RR10888/RR/NCRR NIH HHS; R01 HL074197/HL/NHLBI NIH HHS; R01 HL074197-04/HL/NHLBI NIH HHS; R01 HL74197/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Chondroitin Sulfate Proteoglycans; 0/Lectins, C-Type; 0/dermatan sulfate chondroitin sulfate; 126968-45-4/Versicans; 24967-94-0/Dermatan Sulfate; 9007-28-7/Chondroitin Sulfates; EC 4.2.2.20/Chondroitin ABC Lyase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Biochemical indication for myristoylation-dependent conformational changes in HIV-1 Nef.
Next Document:  Mechanistic insight provided by glutaredoxin within a fusion to redox-sensitive yellow fluorescent p...