| Glycoconjugate expression defines the origin and differentiation pathway of intestinal M-cells. | |
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MedLine Citation:
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PMID: 9313796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intestinal M-cells are specialized epithelial cells located in the domes of the gut-associated lymphoid tissues, which transport antigens from the lumen to the underlying lymphoid tissue, thereby initiating immune reactions. It is assumed that M-cells arise from stem cells in the crypts, from which they migrate to the top of the domes. To study the differentiation pathway of M-cells, we used the rabbit cecal lymphoid patch in which the M-cells express high levels of alpha 1-2-linked fucose and N-acetyl-galactosamine residues in their apical membrane. Dome areas were labeled with fluorescein- and rhodamine-conjugated lectins specific for alpha 1-2-linked fucose and N-acetyl-galactosamine in vivo and in vitro, and were observed with confocal laser scanning microscopy. Ultrathin sections were double labeled with lectin-gold conjugates and the labeling density was quantified by computer-based image analysis. All cecal patch M-cells expressed alpha 1-2-linked fucose and N-acetyl-galactosamine, but the amount of the two saccharides varied considerably depending on the position of the M-cells at the base, flank, or top of the dome. In eight of 18 rabbits studied, radial strips of M-cells with common glycosylation patterns were observed, each strip associated with an individual crypt. Confocal microscopy revealed that lectin-labeled M-cells were not restricted to the dome epithelium but were also detected in the upper third of crypts surrounding the domes. The results show that M-cells are heterogeneous concerning the glycosylation pattern of membrane glycoconjugates. This pattern is modified as the M-cells differentiate and migrate from the base to the top of the dome. Radial strips of M-cells with a common proclivity of glycoconjugate expression suggest that those M-cells that derive from the same crypt have a clonal origin. The presence of (pre-) M-cells in the crypts surrounding the domes indicates that M-cells derive directly from undifferentiated crypt cells and do not develop from differentiated enterocytes. |
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Authors:
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A Gebert; W Posselt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 45 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 1997 Oct |
Date Detail:
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Created Date: 1997-10-23 Completed Date: 1997-10-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1341-50 Citation Subset: IM |
Affiliation:
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Center of Anatomy, Medical School of Hannover, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cecum / cytology, metabolism* Cell Movement Epithelium / metabolism Fluorescent Dyes Glycoconjugates / metabolism* Image Processing, Computer-Assisted Intestinal Mucosa / cytology, metabolism*, physiology Lectins / metabolism Lymphoid Tissue / cytology, metabolism* Male Microscopy, Confocal Microscopy, Electron Plant Lectins* Rabbits |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Glycoconjugates; 0/Lectins; 0/Plant Lectins; 0/Ulex europaeus lectins; 0/Vicia lectins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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