Document Detail


Glycoconjugate expression defines the origin and differentiation pathway of intestinal M-cells.
MedLine Citation:
PMID:  9313796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intestinal M-cells are specialized epithelial cells located in the domes of the gut-associated lymphoid tissues, which transport antigens from the lumen to the underlying lymphoid tissue, thereby initiating immune reactions. It is assumed that M-cells arise from stem cells in the crypts, from which they migrate to the top of the domes. To study the differentiation pathway of M-cells, we used the rabbit cecal lymphoid patch in which the M-cells express high levels of alpha 1-2-linked fucose and N-acetyl-galactosamine residues in their apical membrane. Dome areas were labeled with fluorescein- and rhodamine-conjugated lectins specific for alpha 1-2-linked fucose and N-acetyl-galactosamine in vivo and in vitro, and were observed with confocal laser scanning microscopy. Ultrathin sections were double labeled with lectin-gold conjugates and the labeling density was quantified by computer-based image analysis. All cecal patch M-cells expressed alpha 1-2-linked fucose and N-acetyl-galactosamine, but the amount of the two saccharides varied considerably depending on the position of the M-cells at the base, flank, or top of the dome. In eight of 18 rabbits studied, radial strips of M-cells with common glycosylation patterns were observed, each strip associated with an individual crypt. Confocal microscopy revealed that lectin-labeled M-cells were not restricted to the dome epithelium but were also detected in the upper third of crypts surrounding the domes. The results show that M-cells are heterogeneous concerning the glycosylation pattern of membrane glycoconjugates. This pattern is modified as the M-cells differentiate and migrate from the base to the top of the dome. Radial strips of M-cells with a common proclivity of glycoconjugate expression suggest that those M-cells that derive from the same crypt have a clonal origin. The presence of (pre-) M-cells in the crypts surrounding the domes indicates that M-cells derive directly from undifferentiated crypt cells and do not develop from differentiated enterocytes.
Authors:
A Gebert; W Posselt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  45     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-10-23     Completed Date:  1997-10-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1341-50     Citation Subset:  IM    
Affiliation:
Center of Anatomy, Medical School of Hannover, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cecum / cytology,  metabolism*
Cell Movement
Epithelium / metabolism
Fluorescent Dyes
Glycoconjugates / metabolism*
Image Processing, Computer-Assisted
Intestinal Mucosa / cytology,  metabolism*,  physiology
Lectins / metabolism
Lymphoid Tissue / cytology,  metabolism*
Male
Microscopy, Confocal
Microscopy, Electron
Plant Lectins*
Rabbits
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Glycoconjugates; 0/Lectins; 0/Plant Lectins; 0/Ulex europaeus lectins; 0/Vicia lectins

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