Document Detail

Glycochenodeoxycholate induces rat alveolar epithelial type II cell death and inhibits surfactant secretion in vitro.
MedLine Citation:
PMID:  22569305     Owner:  NLM     Status:  MEDLINE    
Bile acid-induced lung injury has become an important topic for neonatologists after the discovery of a high incidence of infant respiratory distress syndrome complicated from maternal intrahepatic cholestasis. To explore the molecular pathway of bile acid-induced lung injury, we investigated the cytotoxicity of the glycochenodeoxycholate (GCDC) to alveolar epithelial type II cells (AECII), as the main component of bile acid. The results demonstrated that glycochenodeoxycholate induced oxidative stress, mitochondrial damage, and increased caspase activity in the primary cultured AECII. Moreover, ROS scavengers and caspase inhibitors could rescue cell death induced by GCDC in rat AECII. Our results also indicated that GCDC inhibited AECII surfactant secretion. In conclusion, this study suggested that cell death prevention and cell therapy should be considered as therapeutic strategies for infant respiratory distress syndrome complicated from maternal intrahepatic cholestasis.
Hu Zhangxue; Gao Min; Zhao Jinning; Shi Yuan; Wang li; Song Huapei; Li Rui; Zeng Chunyu
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-06
Journal Detail:
Title:  Free radical biology & medicine     Volume:  53     ISSN:  1873-4596     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-18     Completed Date:  2012-10-16     Revised Date:  2013-09-18    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  122-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Department of Pediatrics, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China.
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MeSH Terms
Adenosine Triphosphate / metabolism
Caspase Inhibitors
Caspases / metabolism
Cells, Cultured
Detergents / pharmacology*
Enzyme Inhibitors / pharmacology
Epithelial Cells / cytology,  drug effects*,  metabolism
Fluorescent Antibody Technique
Glycochenodeoxycholic Acid / pharmacology*
L-Lactate Dehydrogenase / metabolism
Oxidative Stress / drug effects*
Pulmonary Alveoli / cytology,  drug effects*,  metabolism
Pulmonary Surfactants / metabolism*
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism
Reg. No./Substance:
0/Caspase Inhibitors; 0/Detergents; 0/Enzyme Inhibitors; 0/Pulmonary Surfactants; 0/Reactive Oxygen Species; 56-65-5/Adenosine Triphosphate; 640-79-9/Glycochenodeoxycholic Acid; EC Dehydrogenase; EC 3.4.22.-/Caspases
Erratum In:
Free Radic Biol Med. 2013 Oct;63:350

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