| Glycine reduces platelet aggregation. | |
| | |
MedLine Citation:
|
PMID: 23135224 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
It has been demonstrated that a wide variety of white blood cells and macrophages (i.e. Kupffer cells, alveolar and peritoneal macrophages and neutrophils) contain glycine-gated chloride channels. Binding of glycine on the receptor stimulates Cl(-) influx causing membrane hyperpolarization that prevents agonist-induced influx of calcium. Since platelet-aggregation is calcium-dependent, this study was designed to test the hypothesis that glycine would inhibit platelet aggregation. Rats were fed diets rich of glycine for 5 days, while controls received isonitrogenous valine. The bleeding time and ADP- and collagen-induced platelet aggregation were measured. Dietary glycine significantly increased bleeding time about twofold compared to valine-treated controls. Furthermore, the amplitude of platelet aggregation stimulated with ADP or collagen was significantly decreased in whole blood drawn from rats fed 2.5 or 5 % dietary glycine by over 50 %. Addition of glycine in vitro (1-10 mM) also blunted rat platelet aggregation in a dose-dependent manner. Strychnine, a glycine receptor antagonist, abrogated the inhibitory effect of glycine on platelet-aggregation in vitro suggesting the glycine works via a glycine receptor. Glycine also blunted aggregation of human platelets. Further, the glycine receptor was detected in both rat and human platelets by western blotting. Based on these data, it is concluded that glycine prevents aggregation of platelets in a dose-dependent manner via mechanisms involving a glycine receptor. |
| | |
Authors:
|
Peter Schemmer; Zhi Zhong; Uwe Galli; Michael D Wheeler; Li Xiangli; Blair U Bradford; Lars O Conzelmann; Dow Forman; José Boyer; Ronald G Thurman |
Related Documents
:
|
1309354 - The conversion of corticosterone to aldosterone is the site of the oxygen sensitivity o... 22213354 - Exposure of the lysine in the gamma chain dodecapeptide of human fibrinogen is not enha... 22984144 - Investigations of human platelet-type 12-lipoxygenase: role of lipoxygenase products in... 1657574 - Inhibition of aldosterone biosynthesis by 18-ethynyl-deoxycorticosterone. 2851314 - Congenital macrothrombocytopenia, leucocyte inclusions, deafness and proteinuria: funct... 9814644 - The potential impact of platelet growth factors in transfusion medicine. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-8 |
Journal Detail:
|
Title: Amino acids Volume: - ISSN: 1438-2199 ISO Abbreviation: Amino Acids Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-8 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9200312 Medline TA: Amino Acids Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of General and Transplant Surgery, Ruprecht-Karls-University, Im Neuenheimer Feld 110, 69120, Heidelberg, Germany, Peter.Schemmer@med.uni-heidelberg.de. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Towards microRNA-based therapeutics for diabetic nephropathy.
Next Document: Vaspin attenuates the apoptosis of human osteoblasts through ERK signaling pathway.