Document Detail


Glycine reduces cadmium-induced alterations in the viability and activation of macrophage U937 cells.
MedLine Citation:
PMID:  19914328     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigates the effect of glycine on cadmium-induced alterations on the viability and activation of the cell line U-937. In this experiment, U-937 cells were pre-treated with 16 microM cadmium (as cadmium chloride). These cadmium-treated cells were later incubated with or without glycine (1-16 microM). After 72 h, it was revealed that glycine significantly (P<0.05) reduced the tendency of cadmium to reduce the viability of the cells. U-937 cells were also treated with phorbol, 12-myristate, 13-acetate to enhance their transition to the macrophage form. Thereafter, the cells were treated with cadmium with or without glycine (1-16 microM). Twenty-four hours later, the supernatants of each cell culture were assessed for the production of tumour necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6), interleukin 1 (IL-1), nitric oxide (NO), and catalase activity as indices of the activation of macrophages. The results show that glycine significantly (P<0.05) reduced the cadmium-induced production of all the markers of the activation of macrophages in a concentration-dependent manner. The findings support the immense antioxidant role of glycine.
Authors:
Tebekeme Okoko; Ejiro Prosper Awhin
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Publication Detail:
Type:  Journal Article     Date:  2009-11-13
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  48     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-04-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  536-8     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Ltd. All rights reserved.
Affiliation:
Biochemistry Programme, Department of Chemical Sciences, Niger Delta University, PMB 71, Wilberforce Island, Bayelsa State, Nigeria. tebebuddy@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / pharmacology*
Biological Markers / analysis,  metabolism
Cadmium Chloride / toxicity*
Catalase / analysis,  metabolism
Cell Survival / drug effects
Culture Media, Conditioned / chemistry,  metabolism
Cytokines / analysis,  metabolism
Dose-Response Relationship, Drug
Environmental Pollutants / toxicity*
Glycine / pharmacology*
Humans
Macrophage Activation / drug effects*,  physiology
Macrophages / drug effects*,  metabolism
Nitric Oxide / analysis,  metabolism
U937 Cells
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Biological Markers; 0/Culture Media, Conditioned; 0/Cytokines; 0/Environmental Pollutants; 10102-43-9/Nitric Oxide; 10108-64-2/Cadmium Chloride; 56-40-6/Glycine; EC 1.11.1.6/Catalase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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