| Glycine reduces cadmium-induced alterations in the viability and activation of macrophage U937 cells. | |
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MedLine Citation:
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PMID: 19914328 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study investigates the effect of glycine on cadmium-induced alterations on the viability and activation of the cell line U-937. In this experiment, U-937 cells were pre-treated with 16 microM cadmium (as cadmium chloride). These cadmium-treated cells were later incubated with or without glycine (1-16 microM). After 72 h, it was revealed that glycine significantly (P<0.05) reduced the tendency of cadmium to reduce the viability of the cells. U-937 cells were also treated with phorbol, 12-myristate, 13-acetate to enhance their transition to the macrophage form. Thereafter, the cells were treated with cadmium with or without glycine (1-16 microM). Twenty-four hours later, the supernatants of each cell culture were assessed for the production of tumour necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6), interleukin 1 (IL-1), nitric oxide (NO), and catalase activity as indices of the activation of macrophages. The results show that glycine significantly (P<0.05) reduced the cadmium-induced production of all the markers of the activation of macrophages in a concentration-dependent manner. The findings support the immense antioxidant role of glycine. |
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Authors:
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Tebekeme Okoko; Ejiro Prosper Awhin |
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Publication Detail:
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Type: Journal Article Date: 2009-11-13 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 48 ISSN: 1873-6351 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 536-8 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
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Biochemistry Programme, Department of Chemical Sciences, Niger Delta University, PMB 71, Wilberforce Island, Bayelsa State, Nigeria. tebebuddy@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology* Biological Markers / analysis, metabolism Cadmium Chloride / toxicity* Catalase / analysis, metabolism Cell Survival / drug effects Culture Media, Conditioned / chemistry, metabolism Cytokines / analysis, metabolism Dose-Response Relationship, Drug Environmental Pollutants / toxicity* Glycine / pharmacology* Humans Macrophage Activation / drug effects*, physiology Macrophages / drug effects*, metabolism Nitric Oxide / analysis, metabolism U937 Cells |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Biological Markers; 0/Culture Media, Conditioned; 0/Cytokines; 0/Environmental Pollutants; 10102-43-9/Nitric Oxide; 10108-64-2/Cadmium Chloride; 56-40-6/Glycine; EC 1.11.1.6/Catalase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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