| Glycine receptors and glycinergic synaptic input at the axon terminals of mammalian retinal rod bipolar cells. | |
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MedLine Citation:
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PMID: 14514876 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated the properties of glycine receptors and glycinergic synaptic inputs at the axon terminals of rod bipolar cells (RBCs) in rats by patch-clamp recording. Glycine currents recorded from isolated axon terminals were larger than those from isolated somata/dendrites; this was confirmed by puffing glycine onto these two regions in retinal slices. The current density at terminal endings was more than one order of magnitude higher than the density at somatic/dendritic regions. Glycine currents from isolated terminals and isolated somata/dendrites showed similar sensitivity to picrotoxinin blockade. Single-channel opening recorded from isolated terminals and somata/dendrites displayed a similar main-state conductance of ~46 pS. Application of glycine effectively suppressed depolarization-evoked increases in intracellular Ca2+ at the terminals. In the presence of GABAA and GABAC antagonists, strychnine-sensitive chloride currents were evoked in RBCs in retinal slices by puffing kainate onto the inner plexiform layer. No such currents were observed if the recorded RBCs did not retain axon terminals or if Ca2+ was replaced by Co2+ in the extracellular solution. The currents displayed discrete miniature-like events, which were partially blocked by tetrodotoxin. Consistent with early studies in the rabbit and mouse, this study demonstrates that glycine receptors are highly concentrated at the axon terminals of rat RBCs. The pharmacological and physiological properties of glycine receptors located in the axon terminal and somatic/dendritic regions, however, appear to be the same. This study provides evidence for the existence of functional glycinergic synaptic input at the axon terminals of RBCs, suggesting that glycine receptors may play a role in modulating bipolar cell synaptic transmission. |
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Authors:
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Jinjuan Cui; Yu-Ping Ma; Stuart A Lipton; Zhuo-Hua Pan |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2003-09-26 |
Journal Detail:
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Title: The Journal of physiology Volume: 553 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-12-15 Completed Date: 2004-08-31 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
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Languages: eng Pagination: 895-909 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Axons / physiology* Dendrites / drug effects, physiology Evoked Potentials / drug effects, physiology* Glycine / pharmacology* Mammals Nerve Endings / physiology Patch-Clamp Techniques Rats Rats, Long-Evans Receptors, Glycine / physiology* Retinal Rod Photoreceptor Cells / cytology*, physiology* Synaptic Transmission / drug effects, physiology* Tetrodotoxin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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1 R01 EY12180/EY/NEI NIH HHS; 1R01 EY05477/EY/NEI NIH HHS; 5 P01 EY04068/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Glycine; 4368-28-9/Tetrodotoxin; 56-40-6/Glycine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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