| Glycine modulates synaptic NR2A- and NR2B-containing NMDA receptor-mediated responses in the rat visual cortex. | |
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MedLine Citation:
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PMID: 18048007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the central nervous system, activation of N-methyl-d-aspartate receptor (NMDA-R) glycine binding sites is a prerequisite for activation of synaptic NMDA-Rs by the excitatory neurotransmitter glutamate. Here we used patch-clamp recordings in transverse slice preparations to study whether the glycine binding site of the NMDA-R saturates and to determine their subunit composition in layer II/III pyramidal neurons of the rat visual cortex. We found that the NMDA-R-mediated component of miniature excitatory postsynaptic currents (mEPSCs) could be potentiated by exogenously applied glycine. Similar results were obtained by exogenously applied d-serine. A specific antagonist for NR2B-NMDA-Rs, Ro 25-6981, reduced NMDA-R-mediated mEPSCs, and glycine with Ro 25-6981 enhanced NMDA-R-mediated mEPSCs. Moreover, Zn2+, an NR2A-NMDA-R antagonist, also reduced NMDA-mediated mEPSCs and glycine with Zn2+ enhanced the NMDA-mediated mEPSCs. Our data indicate that the glycine binding site of synaptic NR2A-containing and NR2B-containing NMDA-Rs does not saturate and that glycine may act as a modulator of NMDA-R-mediated transmission in layer II/III pyramidal neurons of the rat visual cortex. |
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Authors:
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Yan-Hai Li; Tai-Zhen Han |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-12 |
Journal Detail:
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Title: Brain research Volume: 1190 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2008-01-07 Completed Date: 2008-05-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 49-55 Citation Subset: IM |
Affiliation:
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Department of Physiology, School of Medicine, Xi'an Jiaotong University, Zhuque Dajie 205, Xi'an, Shannxi 710061, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Excitatory Postsynaptic Potentials / physiology Glycine / metabolism* Patch-Clamp Techniques Protein Subunits / physiology Pyramidal Cells / metabolism* Rats Rats, Sprague-Dawley Receptors, N-Methyl-D-Aspartate / metabolism* Signal Transduction / physiology Statistics, Nonparametric Synaptic Membranes / metabolism Visual Cortex / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/NR2A NMDA receptor; 0/NR2B NMDA receptor; 0/Protein Subunits; 0/Receptors, N-Methyl-D-Aspartate; 56-40-6/Glycine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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