Document Detail

Glycine modulates synaptic NR2A- and NR2B-containing NMDA receptor-mediated responses in the rat visual cortex.
MedLine Citation:
PMID:  18048007     Owner:  NLM     Status:  MEDLINE    
In the central nervous system, activation of N-methyl-d-aspartate receptor (NMDA-R) glycine binding sites is a prerequisite for activation of synaptic NMDA-Rs by the excitatory neurotransmitter glutamate. Here we used patch-clamp recordings in transverse slice preparations to study whether the glycine binding site of the NMDA-R saturates and to determine their subunit composition in layer II/III pyramidal neurons of the rat visual cortex. We found that the NMDA-R-mediated component of miniature excitatory postsynaptic currents (mEPSCs) could be potentiated by exogenously applied glycine. Similar results were obtained by exogenously applied d-serine. A specific antagonist for NR2B-NMDA-Rs, Ro 25-6981, reduced NMDA-R-mediated mEPSCs, and glycine with Ro 25-6981 enhanced NMDA-R-mediated mEPSCs. Moreover, Zn2+, an NR2A-NMDA-R antagonist, also reduced NMDA-mediated mEPSCs and glycine with Zn2+ enhanced the NMDA-mediated mEPSCs. Our data indicate that the glycine binding site of synaptic NR2A-containing and NR2B-containing NMDA-Rs does not saturate and that glycine may act as a modulator of NMDA-R-mediated transmission in layer II/III pyramidal neurons of the rat visual cortex.
Yan-Hai Li; Tai-Zhen Han
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-12
Journal Detail:
Title:  Brain research     Volume:  1190     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-07     Completed Date:  2008-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  49-55     Citation Subset:  IM    
Department of Physiology, School of Medicine, Xi'an Jiaotong University, Zhuque Dajie 205, Xi'an, Shannxi 710061, PR China.
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MeSH Terms
Excitatory Postsynaptic Potentials / physiology
Glycine / metabolism*
Patch-Clamp Techniques
Protein Subunits / physiology
Pyramidal Cells / metabolism*
Rats, Sprague-Dawley
Receptors, N-Methyl-D-Aspartate / metabolism*
Signal Transduction / physiology
Statistics, Nonparametric
Synaptic Membranes / metabolism
Visual Cortex / cytology,  metabolism*
Reg. No./Substance:
0/NR2A NMDA receptor; 0/NR2B NMDA receptor; 0/Protein Subunits; 0/Receptors, N-Methyl-D-Aspartate; 56-40-6/Glycine

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