| Glycine affects valproate hepatotoxicity. | |
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MedLine Citation:
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PMID: 21469507 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Valproate is a widely used anticonvulsant drug. Valproate is linked to hepatotoxicity which is rare but potentially lethal. The pathomechanism is not yet completely understood. Previous studies have shown a protective effect of glycine on this toxicity in rat hepatocytes (Vance et al., 1994). In the present study we investigated the hepatoxicity of 1-4 mM valproate in combination with glycine through absorption of neutral red from human hepatoma cells (Hep G2) in monolayer cell culture. Cell toxicity was measured by enzyme release with standard photometric test kits. In addition, a histomorphological evaluation was performed. A significant increase in the IC50 value of valproate cytotoxicity after addition of 12 mmol/l glycine was found. The average release of mitochondrial glutamate dehydrogenase as indicator of cell membrane damage decreased after addition of glycine. Cells treated with valproate in combination with 12 mmol/l glycine showed less histomorphological deformation of the nucleus and improved cell adherence. In conclusion a hepatoprotective effect of glycine on valproate-induced toxicity is also given in human hepatocytes. A nonspecific hepatoprotective effect of glycine can be assumed at least in vitro. |
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Authors:
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Lara Junius; Thomas Brune; Michael Deters; Johannes Schulze; Claus-Peter Siegers |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Research communications in molecular pathology and pharmacology Volume: 120-121 ISSN: 1078-0297 ISO Abbreviation: Res. Commun. Mol. Pathol. Pharmacol. Publication Date: 2007-2008 |
Date Detail:
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Created Date: 2011-04-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9437512 Medline TA: Res Commun Mol Pathol Pharmacol Country: United States |
Other Details:
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Languages: eng Pagination: 93-104 Citation Subset: IM |
Affiliation:
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Department of Paediatrics, Otto von Guericke University of Magdeburg, 39120 Magdeburg, Germany. 2.adresse@web.de |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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