| Glyceraldehyde-3-phosphate dehydrogenase of rat erythrocytes has no membrane component. | |
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MedLine Citation:
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PMID: 4041465 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the course of studying mammalian erythrocytes we noted prominent differences in the red cells of the rat. Analysis of ghosts by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis showed that membranes of rat red cells were devoid of band 6 or the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (D-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12). Direct measurements of this enzyme showed that glyceraldehyde-3-phosphate dehydrogenase activity in rat erythrocytes was about 25% of that in human cells; all of the glyceraldehyde-3-phosphate dehydrogenase activity in rat erythrocytes was within the cytoplasm and none was membrane bound; and in the human red cell, about 1/3 of the enzyme activity was within the cytoplasm and 2/3 membrane bound. The release of glyceraldehyde-3-phosphate dehydrogenase from fresh rat erythrocytes immediately following saponin lysis was also determined using the rapid filtration technique recently described. The extrapolated zero-time intercepts of these reactions confirmed that, in the rat erythrocyte, none of the cellular glyceraldehyde-3-phosphate dehydrogenase was membrane bound. Failure of rat glyceraldehyde-3-phosphate dehydrogenase to bind to the membranes of the intact rat erythrocyte seems to be due to cytoplasmic metabolites which interact with the enzyme and render it incapable of binding to the membrane. |
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Authors:
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S K Ballas; H J Kliman; E D Smith |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 831 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1985 Sep |
Date Detail:
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Created Date: 1985-10-30 Completed Date: 1985-10-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 142-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Electrophoresis, Polyacrylamide Gel Erythrocyte Membrane / enzymology Glyceraldehyde-3-Phosphate Dehydrogenases / blood* Hematocrit Hemoglobins / analysis Humans Kinetics Membrane Proteins / analysis Rats |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobins; 0/Membrane Proteins; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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