Document Detail


Glyceraldehyde-3-phosphate dehydrogenase expression varies with age and nutrition status.
MedLine Citation:
PMID:  12714097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme in the glycolytic pathway, and it is a popular internal standard for northern blot analysis. We examined GAPDH expression early in life when feed is either provided or not provided to animals. METHODS: Male broiler chickens were provided a standard starter diet plus Oasis nutritional supplement (fed group; Novus International, St. Louis, MO, USA) or no feed (starved group) for the first 3 d posthatch. Subsequently, the standard starter diet was provided to all chickens between 3 and 7 d posthatch. RNA was extracted from the pectoralis thoracicus, and GAPDH expression was evaluated with quantitative northern analysis. RESULTS: GAPDH expression was significantly (P < 0.05) higher in the fed than in the starved group at 3 d posthatch, suggesting that nutritional manipulations can alter GAPDH transcription. Similarly, GAPDH mRNA levels were significantly (P < 0.05) higher at 7 d posthatch compared with all younger animals, suggesting that GAPDH is developmentally upregulated with advancing age. CONCLUSION: GAPDH expression changes with age and nutrition status in the early posthatch chick, suggesting that GAPDH is not a proper internal standard for muscle studies using quantitative northern analysis.
Authors:
Paul E Mozdziak; Julia J Dibner; Darell W McCoy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nutrition (Burbank, Los Angeles County, Calif.)     Volume:  19     ISSN:  0899-9007     ISO Abbreviation:  Nutrition     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-25     Completed Date:  2003-11-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8802712     Medline TA:  Nutrition     Country:  United States    
Other Details:
Languages:  eng     Pagination:  438-40     Citation Subset:  IM    
Affiliation:
Department of Poultry Science, North Carolina State University, Raleigh, North Carolina 27695, USA. pemozdzi@unity.ncsu.edu
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animal Nutritional Physiological Phenomena*
Animals
Animals, Newborn
Blotting, Northern
Chickens / growth & development,  metabolism*
Food Deprivation
Gene Expression Regulation, Enzymologic
Glyceraldehyde-3-Phosphate Dehydrogenases / genetics,  metabolism*
Male
Nutritional Status*
Pectoralis Muscles / enzymology*
RNA / analysis
Random Allocation
Chemical
Reg. No./Substance:
63231-63-0/RNA; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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