| Glycation of wood frog (Rana sylvatica) hemoglobin and blood proteins: in vivo and in vitro studies. | |
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MedLine Citation:
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PMID: 19540217 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of in vivo freezing and glucose cryoprotectant on protein glycation were investigated in the wood frog, Rana sylvatica. Our studies revealed no difference in the fructoselysine content of blood plasma sampled from control, 27 h frozen and 18 h thawed wood frogs. Glycated hemoglobin (GHb) decreased slightly with 48 h freezing exposure and was below control levels after 7d recovery, while glycated serum albumin was unchanged by 48 h freezing but did increase after 7d of recovery. In vitro exposure of blood lysates to glucose revealed that the GHb production in wood frogs was similar to that of the rat but was lower than in leopard frogs. We conclude that wood frog hemoglobin was glycated in vitro; however, GHb production was not apparent during freezing and recovery when in vivo glucose is highly elevated. It is possible that wood frog blood proteins have different in vivo susceptibilities to glycation. |
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Authors:
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Justin A MacDonald; Thorsten Degenhardt; John W Baynes; Kenneth B Storey |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-06-18 |
Journal Detail:
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Title: Cryobiology Volume: 59 ISSN: 1090-2392 ISO Abbreviation: Cryobiology Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-08-31 Completed Date: 2009-10-29 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: United States |
Other Details:
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Languages: eng Pagination: 223-5 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / metabolism Blood Proteins / metabolism* Freezing Glycosylation Hemoglobins / metabolism* Lysine / analogs & derivatives, metabolism Ranidae |
| Grant Support | |
ID/Acronym/Agency:
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DK-19971/DK/NIDDK NIH HHS; R37 DK019971-34/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Blood Proteins; 0/Hemoglobins; 21291-40-7/fructosyl-lysine; 56-87-1/Lysine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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