Document Detail


Glycated Collagen I (GC) impairs angiogenesis in vitro: a study using an innovative chamber for cell research.
MedLine Citation:
PMID:  17084479     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies of cell-matrix, cell-cell interaction or angiogenesis on different matrices require simultaneous comparison of read-out parameters from differently treated companion cells. The culture conditions (cell number, temperature and volume of culture medium) in different chambers are not completely equalized using conventional methods. It has been reported that cells growing in different environmental conditions may exhibit different proliferation patterns [P. Tracqui, J.W. Liu, O. Collin, J. Clement-Lacroix, E. Planus, Global analysis of endothelial cell line proliferation patterns based on nutrient-depletion models: implications for a standardization of cell proliferation assays, Cell Proliferat. 38(June (3)) (2005) 119-135]. Herein we describe an innovative chamber, which could resolve this problem by significantly improving the standardization of experimental conditions. The chamber was manufactured from a standard cell culture well by its division with a septum into two sections. We utilized the chamber and recently developed topological analysis to examine the effects of glycated matrices on the capillary-like network formation by endothelial cells. Glycated Collagen I resulted in dose-dependent changes to all measured topological characteristics of the capillary-like network, such as the number of branching points, number of meshes and total capillary length. These differences were observed only in neighbored compartments coated with different matrices, but not in the compartments coated with the same matrix. The novel chamber brings an opportunity for better standardization of experimental conditions and simultaneous observation of different experimental groups, reducing the possible effect of any systematic error.
Authors:
Sergey V Brodsky; Roeland M H Merks; Natalia Mendelev; Cara Goo; Jun Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-11-03
Journal Detail:
Title:  Diabetes research and clinical practice     Volume:  76     ISSN:  0168-8227     ISO Abbreviation:  Diabetes Res. Clin. Pract.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-04-02     Completed Date:  2007-07-16     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  8508335     Medline TA:  Diabetes Res Clin Pract     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  463-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques / instrumentation*
Cells, Cultured
Collagen Type I / pharmacology*
Endothelium, Vascular / drug effects,  physiology*
Glycosylation
Humans
Neovascularization, Physiologic / drug effects*
Umbilical Veins / cytology
Grant Support
ID/Acronym/Agency:
DK064863/DK/NIDDK NIH HHS; K01 DK064863/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Collagen Type I
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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