Document Detail

Glyburide enhancement of lactate production in ischemic heart is modified by preconditioning: an in vivo experimental study in pigs by microdialysis technique.
MedLine Citation:
PMID:  8859930     Owner:  NLM     Status:  MEDLINE    
The concentrations of lactate, pyruvate, and adenosine, together with some of their derivatives, were determined in microdialysates from 12 pig hearts, 6 of which were subjected to preconditioning and 40 min of ischemia (index ischemia) and 6 of which were subjected to only 40 min of index ischemia. Two microdialysis probes were inserted in ischemic myocardium. Glyburide (10 mu M) in a modified isotonic Krebs-Ringer phosphate buffer was administered through one of the probes and plain isotonic phosphate buffer was administered through the other. Accordingly, the experimental setup permitted us to study the metabolic effects of glyburide on ischemic myocardium constituting two groups that were either preconditioned or unpreconditioned. The preconditioning effect was validated with area at risk and infarction area measurements in 12 other pigs. We noted no functional differences between the groups. In the unpreconditioned group glyburide infusion resulted in enhanced 60% lactate production during index ischemia. However, preconditioning attenuated the enhancing effect of glyburide on lactate production. The interplay between the effects of glyburide and preconditioning on ischemic myocardium is suggested to be dependent on the different modes of action on the K(+)(ATP) channel.
B G Wikström; G Ronquist; A Waldenström
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  27     ISSN:  0160-2446     ISO Abbreviation:  J. Cardiovasc. Pharmacol.     Publication Date:  1996 May 
Date Detail:
Created Date:  1997-04-10     Completed Date:  1997-04-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  622-8     Citation Subset:  IM    
Department of Cardiology, University Hospital, Uppsala, Sweden.
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MeSH Terms
Adenosine Triphosphate / metabolism
Glyburide / pharmacology*
Hypoglycemic Agents / pharmacology*
Ischemic Preconditioning, Myocardial*
Lactic Acid / metabolism*
Myocardial Ischemia / metabolism*
Potassium Channels / physiology
Reg. No./Substance:
0/Hypoglycemic Agents; 0/Potassium Channels; 10238-21-8/Glyburide; 50-21-5/Lactic Acid; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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