| Glutathione transferases in rat hepatoma cells. Effects of ascites cells on the isoenzyme pattern in liver and induction of glutathione transferases in the tumour cells. | |
| | |
MedLine Citation:
|
PMID: 2920012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Rat hepatoma cells grown intraperitoneally as an ascites tumour were analysed with respect to their contents of cytosolic glutathione transferases. In contrast with normal liver tissue, the hepatoma cells were dominated by the class Pi glutathione transferase 7-7. All the major hepatic enzyme forms were down-regulated to almost undetectable concentrations. Livers of rats bearing ascites-hepatoma cells expressed low, but significant, amounts of protein which, by electrophoretic and immunochemical properties, appeared identical with transferase 7-7. This enzyme is not detectable in normal hepatocytes. Treatment of rats with trans-stilbene oxide induced the expression of transferase 7-7 in the livers of normal rats as well as in hepatoma-cell-bearing animals. In addition, a 2-fold induction of transferase 7-7 was measured in the hepatoma ascites cells. No significant elevation of any other enzyme forms in the hepatoma cells was noted. |
| | |
Authors:
|
M K Tahir; C Guthenberg; B Mannervik |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Biochemical journal Volume: 257 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1989 Jan |
Date Detail:
|
Created Date: 1989-04-06 Completed Date: 1989-04-06 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 215-20 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Cytosol / drug effects, enzymology Glutathione Transferase / metabolism* Isoenzymes / metabolism* Liver Neoplasms, Experimental / enzymology* Rats Stilbenes / pharmacology Tumor Cells, Cultured / drug effects, enzymology |
| Chemical | |
Reg. No./Substance:
|
0/Isoenzymes; 0/Stilbenes; 17619-97-5/stilbene oxide; EC 2.5.1.18/Glutathione Transferase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of heparin on the glia-derived-nexin-thrombin interaction.
Next Document: Physiological significance of the marked increased branching of the glycans of human serotransferrin...