Document Detail

Glutathione redox potential is low and glutathionylated and cysteinylated hemoglobin levels are elevated in maintenance hemodialysis patients.
MedLine Citation:
PMID:  23333585     Owner:  NLM     Status:  Publisher    
Glutathione (GSH), the most abundant intracellular low molecular mass thiol, protects cells from oxidative damage and regulates their function. Available information is inconsistent regarding levels of GSH and its disulfide (GSSG) in maintenance hemodialysis patients (HD). In addition, very limited data are available in HD about the relationship of GSH and GSSG with other measures of thiol metabolism and with the clinical profile. We tested the hypothesis that erythrocyte GSH/GSSG redox potential (E(h)) is lower in HD than in healthy controls (C), and that E(h) correlates with posttranslational thiolation of hemoglobin (Hb) and with standard clinical parameters in HD. In cross-sectional comparison of 33 stable HD and 21 C, we found a net loss of reducing capacity in HD as indicated by low erythrocyte GSH/GSSG E(h) (-257 ± 5.5 vs -270 ± 5.6 mV, P = 0.002). Glutathionylated Hb (HbSSG) was 46% higher in HD than C (19.3 ± 4.80 vs 13.2 ± 2.79 pmol/mg Hb; P = 0.001) and cysteinylated Hb (HbSSCy) was >3-fold higher in HD than C [38.3 (29.0-63.3) vs 11.5 (9.6-17.2) pmol/mg Hb; P = 0.001]. In multiple regression analysis of the HD cases, statistically significant associations were found between the GSH/GSSG E(h) and the blood urea nitrogen (P = 0.001), creatinine (P = 0.015) and normalized protein catabolic rate (P = 0.05), after adjusting for age, race/ethnicity, and etiology of end-stage renal disease. In conclusion, accurate and precise analysis of GSH, GSSG, and mixed disulfides reveals loss of erythrocyte GSH/GSSG E(h,) rise of both HbSSG and HbSSCy, and correlation of these thiols with measures of uremia and dietary protein intake.
Khaled Khazim; Daniela Giustarini; Ranieri Rossi; Darlene Verkaik; John E Cornell; Sue E D Cunningham; Maryam Mohammad; Kara Trochta; Carlos Lorenzo; Franco Folli; Shweta Bansal; Paolo Fanti
Related Documents :
2735335 - Effect of sucralfate and cimetidine on rheumatoid patients with active gastroduodenal l...
19997975 - Gastric neuromuscular pathology in gastroparesis: analysis of full-thickness antral bio...
21879475 - The role of vitamins in the prevention and treatment of thyroid disorders.
11957045 - Clinicopathological study of gastric carcinoma in high- and low-mortality countries: co...
18923245 - Serum gamma-glutamyl transferase activity: new high-risk criteria in acute coronary syn...
8306465 - Postoperative studies on parathyroid hormone secretion in patients operated on for prim...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Translational research : the journal of laboratory and clinical medicine     Volume:  -     ISSN:  1878-1810     ISO Abbreviation:  Transl Res     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101280339     Medline TA:  Transl Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Mosby, Inc. All rights reserved.
Department of Medicine, the University of Texas Health Science Center San Antonio, San Antonio, Tex; Division of Nephrology, Western Galilee Hospital, Nahariya, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Colour and sarcoplasmic protein evaluation of pork following water bath and ohmic cooking.
Next Document:  Engineering cyanobacteria for photosynthetic production of 3-hydroxybutyrate directly from CO2.