Document Detail

Glutathione peroxidase expression and activity in barley root tip after short-term treatment with cadmium, hydrogen peroxide and t-butyl hydroperoxide.
MedLine Citation:
PMID:  23325536     Owner:  NLM     Status:  Publisher    
The purpose of this study was to analyse the alterations of glutathione peroxidase (GPX) expression and activity during the recovery period after a short-term treatment of barley root tip with cadmium (Cd) and hydrogen peroxide (H(2)O(2)). The transcript level of GPX increased as early as 1 h and GPX activity 3 h after short-term treatment independently of Cd concentration. In 15 μM Cd-treated roots, its expression reached a peak within 2 h and sustained until 3 h, after which it gradually declined. After 6 h of short-term Cd treatment, the activity of GPX was the highest in the 15-μM Cd-treated roots. At higher Cd concentrations, the activity of GPX was lower than in 15 μM Cd-treated roots, but still higher than in control roots. A considerable increase in H(2)O(2) production was observed even after only 1 h of short-term exposure of roots to 30 and 60 μM Cd, while after 15 μM Cd exposure, its production increased 3 h after the treatment. Lipid peroxidation increased even 1 h after short-term treatment in a Cd concentration-dependent manner. A considerable decrease of GPX activity was observed after the exposure of roots to H(2)O(2) or t-butyl hydroperoxide in a concentration-dependent manner despite that its expression increased even 1 h after short-term treatment. Presumable, under high acute Cd stress, rapid accumulation of H(2)O(2) leads to the disturbance of basal metabolic processes affecting also GPX activity. In contrast, high GPX activity under moderate Cd stress maintains cell function despite the high rate of H(2)O(2) metabolism in root tip.
Veronika Zelinová; Igor Mistrík; Ján Pavlovkin; Ladislav Tamás
Related Documents :
24328176 - Skp ligands in pd-catalyzed asymmetric allylic amination of mbh adducts: exceptionally ...
24033186 - Visible-light photoredox catalysis: selective reduction of carbon dioxide to carbon mon...
24818256 - Hydrogenated uniform pt clusters supported on porous camno(3) as a bifunctional electro...
24573326 - Improved reversibility in lithium-oxygen battery: understanding elementary reactions an...
818636 - Spectroscopic studies and a structural model for blue copper centers in proteins.
23034736 - Attaching molecular hydrogen to metal cations: perspectives from gas-phase infrared spe...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-17
Journal Detail:
Title:  Protoplasma     Volume:  -     ISSN:  1615-6102     ISO Abbreviation:  Protoplasma     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, 84523, Bratislava, Slovak Republic.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Omega-3 and Omega-6 Fatty Acids and Type 2 Diabetes.
Next Document:  Reactivity to thought-shape fusion in adolescents: the effects of obesity status.