| Glutathione pathway in ethylbenzene metabolism: novel biomarkers of exposure in the rat. | |
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MedLine Citation:
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PMID: 20825968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glutathione pathway was specifically studied in rats exposed by inhalation to a range of ethylbenzene vapours (5-2000 ppm). Urines were collected during exposure (6h) and over the 18 h following the exposure. The potential metabolites coming from either side-chain or ring oxidation were synthesized: 1-, 2-phenylethylmercapturic acids (1-, and 2-PEMA) and 2-, 3- and 4-ethylphenylmercapturic acids (2-, 3-, and 4-EPMA). Their synthesis was fully described and the molecules characterized. Urine samples were analysed using a selective HPLC-fluorescence method. Among the five metabolites, 2-PEMA was never observed in any urine sample. By contrast, 1-PEMA was discovered in its two diastereomeric forms, and it was shown that one of them was mainly present. 2-EPMA, 3-EPMA and 4-EPMA (in the ratio 1:2:6) were also found, and their combined excretion levels were similar to that of 1-PEMA. The atmospheric concentrations and urinary excretions yielded very close correlations which allow us to consider these mercapturic acids as novel ethylbenzene exposure biomarkers. |
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Authors:
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Benoît Cossec; Frédéric Cosnier; Manuella Burgart; Hervé Nunge; Stéphane Grossmann |
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Publication Detail:
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Type: Journal Article Date: 2010-09-09 |
Journal Detail:
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Title: Chemosphere Volume: 81 ISSN: 1879-1298 ISO Abbreviation: Chemosphere Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-08 Completed Date: 2010-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0320657 Medline TA: Chemosphere Country: England |
Other Details:
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Languages: eng Pagination: 1334-41 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Institut National de Recherche et de Sécurité (INRS), Rue du Morvan CS 60027, Vandoeuvre, 54519 Cedex, France. Benoit.cossec@inrs.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzene Derivatives / pharmacokinetics*, toxicity, urine Biological Markers / metabolism, urine Glutathione / metabolism*, urine Inhalation Exposure / analysis* Male Metabolic Networks and Pathways / drug effects* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Benzene Derivatives; 0/Biological Markers; 100-41-4/ethylbenzene; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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