Document Detail

Glutathione pathway in ethylbenzene metabolism: novel biomarkers of exposure in the rat.
MedLine Citation:
PMID:  20825968     Owner:  NLM     Status:  MEDLINE    
Glutathione pathway was specifically studied in rats exposed by inhalation to a range of ethylbenzene vapours (5-2000 ppm). Urines were collected during exposure (6h) and over the 18 h following the exposure. The potential metabolites coming from either side-chain or ring oxidation were synthesized: 1-, 2-phenylethylmercapturic acids (1-, and 2-PEMA) and 2-, 3- and 4-ethylphenylmercapturic acids (2-, 3-, and 4-EPMA). Their synthesis was fully described and the molecules characterized. Urine samples were analysed using a selective HPLC-fluorescence method. Among the five metabolites, 2-PEMA was never observed in any urine sample. By contrast, 1-PEMA was discovered in its two diastereomeric forms, and it was shown that one of them was mainly present. 2-EPMA, 3-EPMA and 4-EPMA (in the ratio 1:2:6) were also found, and their combined excretion levels were similar to that of 1-PEMA. The atmospheric concentrations and urinary excretions yielded very close correlations which allow us to consider these mercapturic acids as novel ethylbenzene exposure biomarkers.
Benoît Cossec; Frédéric Cosnier; Manuella Burgart; Hervé Nunge; Stéphane Grossmann
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Publication Detail:
Type:  Journal Article     Date:  2010-09-09
Journal Detail:
Title:  Chemosphere     Volume:  81     ISSN:  1879-1298     ISO Abbreviation:  Chemosphere     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-08     Completed Date:  2010-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0320657     Medline TA:  Chemosphere     Country:  England    
Other Details:
Languages:  eng     Pagination:  1334-41     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Institut National de Recherche et de Sécurité (INRS), Rue du Morvan CS 60027, Vandoeuvre, 54519 Cedex, France.
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MeSH Terms
Benzene Derivatives / pharmacokinetics*,  toxicity,  urine
Biological Markers / metabolism,  urine
Glutathione / metabolism*,  urine
Inhalation Exposure / analysis*
Metabolic Networks and Pathways / drug effects*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Benzene Derivatives; 0/Biological Markers; 100-41-4/ethylbenzene; 70-18-8/Glutathione

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