| Glutathione metabolism in non-ischemic and postischemic rat hearts in response to an exogenous prooxidant. | |
| | |
MedLine Citation:
|
PMID: 9059989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The objective of the present study was to determine whether mild ischemia hampers the use of tissue concentration of glutathione disulfide (GSSG) and release of GSSG as indices of oxidative stress in postischemic hearts. To this end, the response of the glutathione redox cycle to the prooxidant cumene hydroperoxide (CumOOH) was compared in non-ischemic and postischemic rat hearts perfused in vitro. Perfusion of non-ischemic and postischemic hearts with CumOOH (20-25 microM) for 10 min followed by 20 min of perfusion without CumOOH resulted in similar changes in tissue concentration of GSSG, and similar patterns of GSSG release. The similar response in tissue concentration of GSSG is consistent with the finding that mild ischemia did not affect the formation and reduction of GSSG. While release of GSSG from non-ischemic hearts was solely due to active transport of GSSG, release of GSSG from postischemic hearts was due to both active transport of GSSG and aspecific leakage of GSSG. In conclusion, tissue concentration of GSSG can be reliably used to investigate oxidative stress in postischemic hearts. However, the occurrence of aspecific leakage of GSSG, which is not indicative of oxidative stress, renders release of GSSG from postischemic hearts an unreliable index of oxidative stress. |
| | |
Authors:
|
R J Verbunt; A Van der Laarse |
Related Documents
:
|
12243849 - Oxidative stress and neutrophil activation--the two keystones of ischemia/reperfusion i... 8395629 - Lithospermic acid b as an antioxidant-based protector of cultured ventricular myocytes ... 11934819 - Free-radical production triggered by hyperthermia contributes to heat stress-induced ca... 2571429 - Reperfusion injury and its pharmacologic modification. 17312669 - Myocardial release of non-transferrin-bound iron during cardio-pulmonary bypass surgery. 2529549 - Role of thromboxane receptor antagonists in cardiovascular disease. 1731659 - Calcification of porcine valves: a successful new method of antimineralization. 17155869 - A fiber optic sensor system for control of rate-adaptive cardiac pacemakers and implant... 138359 - Down syndrome with congenital heart malformation. |
Publication Detail:
|
Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Molecular and cellular biochemistry Volume: 167 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 1997 Feb |
Date Detail:
|
Created Date: 1997-05-21 Completed Date: 1997-05-21 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 127-34 Citation Subset: IM |
Affiliation:
|
Department of Cardiology, University Hospital, Leiden, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Benzene Derivatives / metabolism, pharmacokinetics, pharmacology Glutathione / analogs & derivatives, metabolism* Glutathione Disulfide Heart / drug effects Male Myocardial Ischemia / drug therapy, metabolism* Myocardium / metabolism Oxidants / pharmacology* Oxidation-Reduction Perfusion Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
|
0/Benzene Derivatives; 0/Oxidants; 27025-41-8/Glutathione Disulfide; 70-18-8/Glutathione; 80-15-9/cumene hydroperoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Inhibition of ubiquitous mitochondrial creatine kinase expression in HeLa cells by an antisense olig...
Next Document: Effect of dexamethasone on T-cell receptor/CD3 expression.