Document Detail


Glutathione exposes sequential IgE-epitopes in ovomucoid relevant in persistent egg allergy.
MedLine Citation:
PMID:  23349099     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
SCOPE: Patients with persistent egg allergy have more immunoglobulin E (IgE) against sequential than conformational epitopes of ovomucoid (OVO). Here, we aimed to identify compounds capable to render sequential epitopes in egg. METHODS AND RESULTS: Glutathione was used for in vitro reduction of OVO and circular dichroism analyses were performed. Glutathione reduced OVO in a concentration-dependent manner. Egg white was analyzed for reduced proteins with a thiol probe and by MALDI-TOF/TOF. In unprocessed total egg white, several reduced proteins were detected by the thiol probe, among them reduced ovalbumin could be confirmed with MS analyses. Egg-allergics or sensitized controls were tested serologically (n = 19) for IgE against native and reduced OVO and in skin prick tests (n = 9). More patients had IgE against reduced than native OVO in Western blots. In skin prick test, five out of seven persistent egg-allergics and none of the controls reacted with reduced OVO. CONCLUSION: Reduced egg proteins are present in natural egg white. Glutathione, which is present in egg and furthermore is used as texture-improving additive in processed food, is capable of reducing OVO. Patients with persistent egg allergy reacted rather to reduce the native OVO. Hence, our data indicate that reduction is a novel natural and processing-associated principle, which contributes to the allergenicity of food.
Authors:
Franziska Roth-Walter; Philipp Starkl; Torsten Zuberbier; Karin Hummel; Karin Nöbauer; Ebrahim Razzazi-Fazeli; Richard Brunner; Isabella Pali-Schöll; Janis Kinkel; Ferdinand Felix; Erika Jensen-Jarolim; Tamar Kinaciyan
Related Documents :
19334749 - Phytoalexin-enriched functional foods.
16944639 - The arctic in an earth system context: from brake to accelerator of change.
24633249 - Carotenoids of sea angels clione limacina and paedoclione doliiformis from the perspect...
18756399 - Fabrication, functionalization, and application of electrospun biopolymer nanofibers.
19941619 - Functional foods for health promotion: state-of-the-science on dietary flavonoids. exte...
24444969 - Vaccinium corymbosum l. (blueberry) extracts exhibit protective action against cadmium ...
15462309 - Natural history of the lizard enyalius brasiliensis (lesson, 1828) (leiosauridae) from ...
3924019 - Nutrient requirements in various clinical conditions.
8525579 - Effects of the sucking louse (linognathus vituli) on the grooming behaviour of housed c...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  Molecular nutrition & food research     Volume:  -     ISSN:  1613-4133     ISO Abbreviation:  Mol Nutr Food Res     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101231818     Medline TA:  Mol Nutr Food Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Comparative Medicine, Messerli Research Institute, University of Veterinary Medicine Vienna, Vienna, Medical University of Vienna and University of Vienna, Austria.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evidence for distinct genetic effects associated with response to 35% CO?.
Next Document:  Demineralized bone matrix and human cancellous bone enhance fixation of porous-coated titanium impla...