Document Detail


Glutathione adducts, not carbamylated lysines, are the major modification of lens alpha-crystallins from renal failure patients.
MedLine Citation:
PMID:  7576086     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
alpha-Crystallins from the water-soluble and the water-insoluble, guanidine-soluble portions of lenses from four renal failure patients and two normal donors of similar age were isolated and enzymatically digested into peptides. Molecular weights of the peptides, determined by fast atom bombardment mass spectrometry, indicated modifications specifically associated with renal failure. The only modifications observed in the alpha-crystallins from renal failure patients, but not in the normal old lenses, were glutathione adducts to Cys 131 and Cys 142. These adducts were present in the lenses of all four renal failure patients, but not in the two normal old lenses. The four lenses from the renal failure patients were searched for evidence of carbamylation at lysyl or cysteinyl residues: carbamylation was not detected. Because the same mass spectrometric methods had previously demonstrated sufficient sensitivity and specificity to detect as little as 5% modification in the examination of in vitro carbamylated bovine lenses, these results indicated that carbamylation is not a major modification of the lens alpha-crystallins of renal failure patients.
Authors:
J B Smith; G A Shun-Shin; Y Sun; L R Miesbauer; Z Yang; Z Yang; X Zhou; J Schwedler; D L Smith
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of protein chemistry     Volume:  14     ISSN:  0277-8033     ISO Abbreviation:  J. Protein Chem.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-11-28     Completed Date:  1995-11-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8217321     Medline TA:  J Protein Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  179-88     Citation Subset:  IM    
Affiliation:
Department of Medicinal Chemistry and Pharmacognosy, Purdue University, West Lafayette, Indiana 47907, USA.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Aging / metabolism
Carbamates / metabolism*
Crystallins / chemistry,  metabolism*
Female
Glutathione / metabolism*
Guanidine
Guanidines / chemistry
Humans
Kidney Failure / metabolism*
Lysine / metabolism*
Male
Middle Aged
Solubility
Spectrometry, Mass, Fast Atom Bombardment
Water / chemistry
Grant Support
ID/Acronym/Agency:
EY RO1 07609/EY/NEI NIH HHS; GM 08298/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Carbamates; 0/Crystallins; 0/Guanidines; 113-00-8/Guanidine; 56-87-1/Lysine; 70-18-8/Glutathione; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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