Document Detail


Glutathione Modulates the Toxicity of, but is not a Biologically Relevant Reductant for, the Pseudomonas aeruginosa Redox Toxin Pyocyanin.
MedLine Citation:
PMID:  21255639     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Pyocyanin is an important redox toxin produced by the common human pathogen Pseudomonas aeruginosa. It generates reactive oxygen species (ROS) that alter intracellular redox status and cell function. Reducing equivalents for pyocyanin are provided by intracellular NAD(P)H and, it has been reported, glutathione (GSH). Cellular GSH levels are at least 1-2 orders of magnitude greater than NAD(P)H therefore GSH should represent the major reductant for pyocyanin and potentiate its toxicity. Paradoxically, GSH has been found to inhibit pyocyanin toxicity in cellular models. This study was undertaken to evaluate the potential of GSH as a biologically relevant reductant for pyocyanin. Using spectrophotometry, under aerobic conditions pyocyanin readily oxidized NADPH while oxidation of GSH could not be detected. Under anaerobic conditions pyocyanin was reduced by NADPH while reduction by GSH could not be detected. Reduction of molecular oxygen and the formation of ROS readily proceeded in the presence of pyocyanin and NADPH while GSH was without effect. Finally, exposure of normal human dermal fibroblasts to sub-cytotoxic concentrations of pyocyanin did not lead to depletion of endogenous GSH while exogenous GSH provided protection against the senescence-inducing effects of the toxin. In summary, GSH does not reduce pyocyanin under physiologically relevant conditions or contribute to pyocyanin toxicity. However, GSH does provide protection against the deleterious effects of this important bacterial toxin towards mammalian cells.
Authors:
Michael Muller
Related Documents :
16320989 - Facile, scalable, regioselective synthesis of c3v c60h18 using organic polyamines.
23578339 - Isolation and identification of lignans from caulis bambusae in taenia with antioxidant...
22053949 - Iron-catalyzed oxidation of tertiary amines: synthesis of β-1,3-dicarbonyl aldehydes b...
22148289 - De novo synthesis of l-colitose and l-rhodinose building blocks.
23778119 - New resveratrol oligomer derivatives from the roots of rheum lhasaense.
12234349 - An arsenic(iii)-oxidizing bacterial population: selection, characterization, and perfor...
21189169 - Hypercholesterolemia, oxidative stress and gender dependence in children.
20669939 - Fragmentation of trifluoromethylated alkenes and acetylenes by n,n-binucleophiles. synt...
25157959 - Tetraoxanes as a new class of efficient herbicides comparable to commercial products.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-18
Journal Detail:
Title:  Free radical biology & medicine     Volume:  -     ISSN:  1873-4596     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2010. Published by Elsevier Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Crosstalk between PDGF-induced Nox4 activation and MUC8 gene overexpression in Human Airway Epitheli...
Next Document:  Changes in reactive oxygen species begin early during replicative aging of Saccharomyces cerevisiae ...