Document Detail


Glutamine is required for persistent epileptiform activity in the disinhibited neocortical brain slice.
MedLine Citation:
PMID:  20107056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The neurotransmitter glutamate is recycled through an astrocytic-neuronal glutamate-glutamine cycle in which synaptic glutamate is taken up by astrocytes, metabolized to glutamine, and transferred to neurons for conversion back to glutamate and subsequent release. The extent to which neuronal glutamate release is dependent upon this pathway remains unclear. Here we provide electrophysiological and biochemical evidence that in acutely disinhibited rat neocortical slices, robust release of glutamate during sustained epileptiform activity requires that neurons be provided a continuous source of glutamine. We demonstrate that the uptake of glutamine into neurons for synthesis of glutamate destined for synaptic release is not strongly dependent on the system A transporters, but requires another unidentified glutamine transporter or transporters. Finally, we find that the attenuation of network activity through inhibition of neuronal glutamine transport is associated with reduced frequency and amplitude of spontaneous events detected at the single-cell level. These results indicate that availability of glutamine influences neuronal release of glutamate during periods of intense network activity.
Authors:
Hiroaki Tani; Chris G Dulla; John R Huguenard; Richard J Reimer
Related Documents :
11011006 - Short-term ethanol exposure alters calbindin d28k and glial fibrillary acidic protein i...
15314966 - Histological changes in neurons of the globus pallidus after experimental administratio...
20880506 - Astrocytes and epilepsy.
6873216 - The microenvironment of the spinal ganglion neuron in the rat during aging.
22210466 - The nucleus retroambiguus as possible site for inspiratory rhythm generation caudal to ...
11018786 - Afferents from the auditory thalamus synapse on inhibitory interneurons in the lateral ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  30     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-28     Completed Date:  2010-07-08     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1288-300     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Action Potentials / drug effects,  physiology*
Animals
Carrier Proteins / drug effects,  metabolism
Epilepsy / metabolism*,  physiopathology
Glutamic Acid / metabolism
Glutamine / metabolism*
Neocortex / drug effects,  metabolism*,  physiopathology
Nerve Net / drug effects,  metabolism,  physiopathology
Neural Inhibition / drug effects,  physiology*
Neural Pathways / metabolism,  physiopathology
Neurons / drug effects,  metabolism*
Organ Culture Techniques
Presynaptic Terminals / drug effects,  metabolism
Rats
Rats, Sprague-Dawley
Synaptic Transmission / drug effects,  physiology
Grant Support
ID/Acronym/Agency:
K02 NS045634/NS/NINDS NIH HHS; K02 NS045634-05/NS/NINDS NIH HHS; NS007280/NS/NINDS NIH HHS; NS012151/NS/NINDS NIH HHS; NS045634/NS/NINDS NIH HHS; P01 NS012151/NS/NINDS NIH HHS; P01 NS012151-34/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/glutamine transport proteins; 0RH81L854J/Glutamine; 3KX376GY7L/Glutamic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A longitudinal examination of the neurodevelopmental impact of prenatal immune activation in mice re...
Next Document:  Inositol 1,4,5-trisphosphate receptor and dSTIM function in Drosophila insulin-producing neurons reg...