Document Detail

Glutamine protects function and improves preservation of small bowel segments.
MedLine Citation:
PMID:  9441799     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Improved organ preservation is essential for the success of small bowel transplantation. Small bowel is usually preserved in UW (University of Wisconsin) solution which does not contain glutamine (Gln), the principal fuel for the enterocyte. We hypothesized that Gln-supplemented UW would improve mucosal function and structure of cold preserved small intestine. MATERIALS AND METHODS: Jejunum (40 cm) was harvested from Lewis rats and preserved for 18 hr at 4 degrees C in saline; UW solution only; UW with 1, 2, or 4% Gln; and UW containing 1, 2, or 4% isonitrogenous balanced nonessential amino acids (NEAA). 14C glucose transport, mucosal protein, mucosal maltase and alkaline phosphatase, jejunal villous height, and histologic damage were measured. RESULTS: UW with 2% Gln significantly increased glucose transport and mucosal protein when compared to the 2% NEAA and UW-only groups. Two percent Gln significantly decreased histologic damage of jejunum following cold preservation. Increasing Gln to 4% did not significantly increase its efficacy when compared to the UW with 2% Gln group. There were no significant differences in the activities of mucosal maltase and alkaline phosphatase among the various treatment groups. CONCLUSIONS: The addition of Gln, optimally provided at a concentration of 2%, to UW solution may protect the preserved small bowel segments from cold ischemic injury and improve mucosal function.
K Sasaki; J O Park; A Bain; K J Reilly; W T Adamson; S Koide; W Zhang; J L Rombeau
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of surgical research     Volume:  73     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-02-11     Completed Date:  1998-02-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  90-4     Citation Subset:  IM    
Copyright Information:
Copyright 1997 Academic Press.
The Harrison Department of Surgical Research, Hospital of the University of Pennsylvania, 36th Street and Hamilton Walk, Philadelphia, Pennsylvania 19104, USA.
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MeSH Terms
Alkaline Phosphatase / metabolism
Biological Transport
Cold Temperature
Glucose / metabolism
Glutamine / pharmacology*
Intestinal Mucosa / anatomy & histology,  physiology
Intestine, Small / anatomy & histology,  physiology*,  transplantation*
Jejunum / anatomy & histology,  physiology
Organ Preservation*
Organ Size
Proteins / metabolism
Rats, Inbred Lew
alpha-Glucosidases / metabolism
Reg. No./Substance:
0/Proteins; 50-99-7/Glucose; 56-85-9/Glutamine; EC Phosphatase; EC

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