Document Detail


Glutamine-enriched total parenteral nutrition maintains intestinal interleukin-4 and mucosal immunoglobulin A levels.
MedLine Citation:
PMID:  11011781     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Total parenteral nutrition (TPN) prevents progressive malnutrition but fails to maintain intestinal gut-associated lymphoid tissue (GALT) or established respiratory antiviral or antibacterial mucosal immunity. Our previous work demonstrated that decreases in intestinal immunoglobulin A (IgA) were associated with decreases in Th2-type IgA-stimulating cytokines, interleukin (IL)-4 and IL-10. Because glutamine supplementation of TPN partially preserves respiratory defenses and normalizes GALT, we investigated the ability of parenteral glutamine to normalize respiratory and intestinal IgA levels and measured Th2 cytokines in intestinal homogenates. METHODS: Animals were cannulated and randomly assigned to receive chow (n = 17), TPN (n = 18), or an isonitrogenous, isocaloric TPN solution formulated by removing the appropriate amount of amino acids and replacing them with 2% glutamine (n = 18) for 5 days. Respiratory tract and intestinal washings were obtained for IgA and the intestine homogenized and analyzed for IL-4 and IL-10. RESULTS: TPN decreased intestinal and respiratory IgA in association with decreases in intestinal IL-4 and IL-10 compared with chow-fed animals. Glutamine significantly improved respiratory and intestinal IgA levels, significantly improved IL-4 compared with TPN animals, and maintained IL-10 levels midway between chow-fed and TPN animals. CONCLUSIONS: Glutamine-enriched TPN preserved both extraintestinal and intestinal IgA levels and had a normalizing effect on Th2-type IgA-stimulating cytokines.
Authors:
K A Kudsk; Y Wu; K Fukatsu; B L Zarzaur; C D Johnson; R Wang; M K Hanna
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  JPEN. Journal of parenteral and enteral nutrition     Volume:  24     ISSN:  0148-6071     ISO Abbreviation:  JPEN J Parenter Enteral Nutr     Publication Date:    2000 Sep-Oct
Date Detail:
Created Date:  2000-12-27     Completed Date:  2001-03-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7804134     Medline TA:  JPEN J Parenter Enteral Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  270-4; discussion 274-5     Citation Subset:  IM    
Affiliation:
University of Tennessee, Memphis, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Glutamine / administration & dosage,  pharmacology*
Immunoglobulin A / analysis,  drug effects*
Interleukin-10 / analysis
Interleukin-4 / analysis*
Intestinal Mucosa / immunology*
Male
Mice
Mice, Inbred ICR
Parenteral Nutrition, Total*
Random Allocation
Respiratory System / immunology*
Grant Support
ID/Acronym/Agency:
5 R01 GM53439/GM/NIGMS NIH HHS; T35-DK07405/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Immunoglobulin A; 130068-27-8/Interleukin-10; 207137-56-2/Interleukin-4; 56-85-9/Glutamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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