Document Detail


Glutamine as a precursor to N-terminal pyrrolid-2-one-5-carboxylic acid in mouse immunoglobulin lambda-type light chains. Amino acid-sequence variability at the N-terminal extra piece of lambda-type light-chain precursors.
MedLine Citation:
PMID:  411485     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mRNA molecules coding for three mouse immunoglobulin lambda-type light (L) chains (MOPC-104E lambda(1), RPC-20 lambda(1), MOPC-315 lambda(2)) programme the cell-free synthesis of precursors larger than the mature proteins. Radioactive amino acid-sequence analyses of each of the three precursors labelled with [(3)H]alanine, [(3)H]serine, [(3)H]glutamine, [(3)H]glutamic acid and [(3)H]threonine showed that an extra piece, at least 18 residues long, is linked to the N-terminus of the mature L-chains. The N-terminal extra-peptide segment may be 19 residues long, since analyses of precursors labelled with [(35)S]methionine indicated an additional N-terminal methionine residue which was recovered in low yields. Presumably this is the initiator methionine, which is known to be short lived in eukaryotes. The mature forms of MOPC-104E, RPC-20 and MOPC-315 lambda L-chains are blocked at the N-termini by pyrrolid-2-one-5-carboxylic acid (pyroglutamic acid). Sequence analyses of precursors labelled with [(3)H]glutamine and [(3)H]glutamic acid showed incorporation only of glutamine in a position that matches with the position of pyrrolid-2-one-5-carboxylic acid in the mature forms of all three precursors, and incorporation of glutamic acid in other positions. The data showed the absence of glutamine-glutamic acid interconversion, since the radioactive peaks obtained from either (3)H-labelled amino acid were discrete, and free from cross-contamination. These results prove that glutamine is the precursor amino acid of pyrrolid-2-one-5-carboxylic acid at the N-termini of the mature MOPC-104E lambda(1), RPC-20 lambda(1) and MOPC-315 lambda(2) L-chains. Thus the formation of pyrrolid-2-one-5-carboxylic acid by cyclization of glutamine is a post-translational event which occurs after, or concomitant with, cleavage of the extra piece from the precursor to yield the mature L-chain. The variable (V) regions (110 amino acid residues) of mouse lambda L-chains are quite similar: when compared with that of MOPC-104E lambda(1) chain, the V-region of RPC-20 lambda(1) chain differs in one residue, and the V-region of MOPC-315 lambda(2) chain differs in 11 residues. The partial sequence data show that the N-terminal extra pieces of the two lambda(1) L-chain precursors have, so far, identical partial sequences; the extra piece of the lambda(2) L-chain precursor differs from these in at least three out of 19 positions.
Authors:
Y Burstein; I Schechter
Related Documents :
25077565 - Cloning, characterization of a cold-adapted endo-1,5-α-l-arabinanase from paenibacillu...
1175625 - Synthesis of apamin, a neurotoxic peptide from bee venom.
10794525 - Cloning and expression of the mouse deoxyuridine triphosphate nucleotidohydrolase gene:...
22976975 - The [(cp)m(co)(3) ] (m=re, (99m) tc) building block for imaging agents and bioinorganic...
7462015 - Lysosomal enzymes in oocytes of the mongolian gerbil, meriones unguiculatus.
20633905 - Mercury concentrations and omega-3 fatty acids in fish and shrimp: preferential consump...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  165     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-12-29     Completed Date:  1977-12-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  347-54     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Glutamine / metabolism*
Immunoglobulin Light Chains / biosynthesis*
Immunoglobulin lambda-Chains / biosynthesis*
Mice
Myeloma Proteins / biosynthesis
Peptide Chain Termination, Translational
Pyrrolidinones / metabolism*
Pyrrolidonecarboxylic Acid / metabolism*
RNA, Messenger
Chemical
Reg. No./Substance:
0/Immunoglobulin Light Chains; 0/Immunoglobulin lambda-Chains; 0/Myeloma Proteins; 0/Pyrrolidinones; 0/RNA, Messenger; 56-85-9/Glutamine; 98-79-3/Pyrrolidonecarboxylic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fragments produced by digestion of human immunoglobulin G subclasses with pepsin in urea.
Next Document:  The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-al...