Document Detail


Glutamine analogs promote cytoophidium assembly in human and Drosophila cells.
MedLine Citation:
PMID:  21930098     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CTP synthase is compartmentalized within a subcellular structure, termed the cytoophidium, in a range of organisms including bacteria, yeast, fruit fly and rat. Here we show that CTP synthase is also compartmentalized into cytoophidia in human cells. Surprisingly, the occurrence of cytoophidia in human cells increases upon treatment with a glutamine analog 6-diazo-5-oxo-l-norleucine (DON), an inhibitor of glutamine-dependent enzymes including CTP synthase. Experiments in flies confirmed that DON globally promotes cytoophidium assembly. Clonal analysis via CTP synthase RNA interference in somatic cells indicates that CTP synthase expression level is critical for the formation of cytoophidia. Moreover, DON facilitates cytoophidium assembly even when CTP synthase level is low. A second glutamine analog azaserine also promotes cytoophidum formation. Our data demonstrate that glutamine analogs serve as useful tools in the study of cytoophidia.
Authors:
Kangni Chen; Jing Zhang; Ömür Yilmaz Tastan; Zillah Anne Deussen; Mayte Yu-Yin Siswick; Ji-Long Liu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-08-17
Journal Detail:
Title:  Journal of genetics and genomics = Yi chuan xue bao     Volume:  38     ISSN:  1673-8527     ISO Abbreviation:  J Genet Genomics     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-20     Completed Date:  2012-04-09     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  101304616     Medline TA:  J Genet Genomics     Country:  China    
Other Details:
Languages:  eng     Pagination:  391-402     Citation Subset:  IM    
Copyright Information:
Copyright © 2011. Published by Elsevier Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Azaserine / analogs & derivatives,  pharmacology
Carbon-Nitrogen Ligases / antagonists & inhibitors,  deficiency,  genetics,  metabolism
Cell Compartmentation / drug effects*
Cell Cycle / drug effects
Diazooxonorleucine / analogs & derivatives,  pharmacology*
Drosophila melanogaster / cytology*,  drug effects*,  enzymology,  metabolism
Enzyme Inhibitors / chemistry,  pharmacology*
Female
Glutamine / analogs & derivatives*
HeLa Cells
Humans
Intracellular Space / drug effects*,  enzymology,  metabolism*
Male
Oogenesis / drug effects
RNA Interference
Grant Support
ID/Acronym/Agency:
MC_U137788471//Medical Research Council; R01-GM084947/GM/NIGMS NIH HHS; //Medical Research Council
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 03J0H273KZ/Diazooxonorleucine; 0RH81L854J/Glutamine; 87299V3Q9W/Azaserine; EC 6.3.-/Carbon-Nitrogen Ligases; EC 6.3.4.2/CTP synthetase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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