| Glutamine analogs promote cytoophidium assembly in human and Drosophila cells. | |
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MedLine Citation:
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PMID: 21930098 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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CTP synthase is compartmentalized within a subcellular structure, termed the cytoophidium, in a range of organisms including bacteria, yeast, fruit fly and rat. Here we show that CTP synthase is also compartmentalized into cytoophidia in human cells. Surprisingly, the occurrence of cytoophidia in human cells increases upon treatment with a glutamine analog 6-diazo-5-oxo-l-norleucine (DON), an inhibitor of glutamine-dependent enzymes including CTP synthase. Experiments in flies confirmed that DON globally promotes cytoophidium assembly. Clonal analysis via CTP synthase RNA interference in somatic cells indicates that CTP synthase expression level is critical for the formation of cytoophidia. Moreover, DON facilitates cytoophidium assembly even when CTP synthase level is low. A second glutamine analog azaserine also promotes cytoophidum formation. Our data demonstrate that glutamine analogs serve as useful tools in the study of cytoophidia. |
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Authors:
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Kangni Chen; Jing Zhang; Omür Yilmaz Tastan; Zillah Anne Deussen; Mayte Yu-Yin Siswick; Ji-Long Liu |
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Publication Detail:
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Type: Journal Article Date: 2011-08-17 |
Journal Detail:
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Title: Journal of genetics and genomics = Yi chuan xue bao Volume: 38 ISSN: 1673-8527 ISO Abbreviation: J Genet Genomics Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-09-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101304616 Medline TA: J Genet Genomics Country: China |
Other Details:
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Languages: eng Pagination: 391-402 Citation Subset: IM |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ltd. |
Affiliation:
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Medical Research Council Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, United Kingdom. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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