Document Detail


Glutamate transporters in the central nervous system of a pond snail.
MedLine Citation:
PMID:  19937812     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies on glutamate (GLU) and its receptors in the pond snail Lymnaea stagnalis have suggested that GLU functions as a neurotransmitter in various behaviors, particularly for generation of feeding rhythm. The uptake mechanism of GLU is not yet known in Lymnaea. In the present study, we characterized the GLU transporters and examined their functions in the feeding circuits of the central nervous system (CNS) in Lymnaea. First, measurement of the accumulation of (3)H-labeled GLU revealed the presence of GLU transport systems in the Lymnaea CNS. The highest accumulation rate was observed in the buccal ganglia, supporting the involvement of GLU transport systems in feeding behavior. Second, we cloned two types of GLU transporters from the Lymnaea CNS, the excitatory amino acid transporter (LymEAAT) and the vesicular GLU transporter (LymVGLUT). When we compared their amino acid sequences with those of mammalian EAATs and VGLUTs, we found that the functional domains of both types are well conserved. Third, in situ hybridization revealed that the mRNAs of LymEAAT and LymVGLUT are localized in large populations of nerve cells, including the major feeding motoneurons in the buccal ganglia. Finally, we inhibited LymEAAT and found that changes in the firing patterns of the feeding motoneurons that have GLUergic input were similar to those obtained following stimulation with GLU. Our results confirmed the presence of GLU uptake systems in the Lymnaea CNS and showed that LymEAAT is required for proper rhythm generation, particularly for generation of the feeding rhythm.
Authors:
Dai Hatakeyama; Koichi Mita; Suguru Kobayashi; Hisayo Sadamoto; Yutaka Fujito; L?szl? Hiripi; K?roly Elekes; Etsuro Ito
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  88     ISSN:  1097-4547     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-01     Completed Date:  2010-06-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1374-86     Citation Subset:  IM    
Copyright Information:
(c) 2009 Wiley-Liss, Inc.
Affiliation:
Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Sanuki, Japan.
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MeSH Terms
Descriptor/Qualifier:
Action Potentials
Amino Acid Sequence
Animals
Central Nervous System / metabolism
Ganglia, Invertebrate / metabolism
Glutamate Plasma Membrane Transport Proteins / genetics,  metabolism*
Glutamic Acid / metabolism
In Situ Hybridization
Lymnaea / metabolism*
Microelectrodes
Molecular Sequence Data
Motor Neurons / metabolism
Neurons / metabolism
Potassium / metabolism
RNA, Messenger / metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tritium
Vesicular Glutamate Transport Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Glutamate Plasma Membrane Transport Proteins; 0/RNA, Messenger; 0/Vesicular Glutamate Transport Proteins; 10028-17-8/Tritium; 56-86-0/Glutamic Acid; 7440-09-7/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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