Document Detail


Glutamate release in parabrachial nucleus and baroreflex alterations after vagal afferent activation.
MedLine Citation:
PMID:  9176358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The parabrachial nucleus (PBN) is a regulatory nucleus that relays visceral information from the brain stem to the cortex. Immunohistochemical studies have shown that the levels of various neuropeptides in the PBN were changed after visceral afferent activation. Because the major transmitter relaying visceral information through the PBN is glutamate, the present study asked if glutamate release into the PBN also was changed after vagal afferent activation in anesthetized male rats. The distally crushed vagus was stimulated (50 Hz, 1-2 mA, 1 s on, 2 s off) for 2 h. Dialysates of the PBN were collected every 30 min and assayed for glutamate using high-performance liquid chromatography. Extracellular glutamate concentrations were reduced during the vagal stimulation, increased fourfold compared with prestimulated levels after the stimulation was terminated, and returned to prestimulated levels over the next 2 h poststimulation. These changes were not due to alterations in blood pressure because sodium nitroprusside infusion for the same interval resulted in a similar hypotension, but increased glutamate release. Phenylephrine and sodium nitroprusside were infused intravenously to measure the cardiac baroreflex before, during, and after vagal stimulation. The pressor (but not the depressor) response was elevated during the period of enhanced glutamate release, and the baroreflex curve was shifted to the right (increased threshold) without a change in gain. These changes in the cardiac baroreflex were reduced, but still seen, when the PBNs were dialyzed bilaterally with the glutamate antagonists MK-801 and 6-cyano-7-nitroquinoxaline-2,3-dione. Thus visceral activation alters glutamate release in the PBN and has enduring effects on cardiac baroreflex function.
Authors:
T M Saleh; L G Bauce; Q J Pittman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  272     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-06-27     Completed Date:  1997-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R1631-40     Citation Subset:  IM    
Affiliation:
Neuroscience Research Group, University of Calgary, Alberta, Canada.
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MeSH Terms
Descriptor/Qualifier:
Afferent Pathways
Animals
Baroreflex*
Blood Pressure
Electric Stimulation
Glutamates / metabolism*
Heart Rate
Hypertension / physiopathology
Male
Nitroprusside / pharmacology
Pons / physiology*
Rats
Rats, Sprague-Dawley
Vagus Nerve / physiology*
Chemical
Reg. No./Substance:
0/Glutamates; 15078-28-1/Nitroprusside

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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