Document Detail

Glutamate exocrine dynamics augmented by plasma glutamine and the distribution of amino acid transporters of the rat pancreas.
MedLine Citation:
PMID:  20610855     Owner:  NLM     Status:  MEDLINE    
The nutritional and physiological roles of amino acid (AA)s have been investigated for individual organs. In the current study, we focused on the dynamics of glutamate and transport systems in the pancreas. We employed original procedures to obtain rat pancreatic juice (PJ) subjected to intravenous administration of alanyl-glutamine (AG) for AA analysis. The pancreatic expressions of the transporters were evaluated by immunohistochemistry. We found that glutamate was secreted into the PJ in the basal state. The intravenous administration of AG increased the concentration and total amount of glutamate excreted into the PJ. In terms of the transport systems, L-type AA transporter (LAT1) was identified exclusively in the islet cells. Glutamate transporter 1 (GLT1), glutamate-aspartate transporter (GLAST), vesicular glutamate transporter 1 (VGUT1) and cystine/glutamic acid transporter (xCT) were found in the islet cells. xCT was identified in the duct cells as well, but was not accompanied by the expression of 4F2 heavy chain (4F2hc) staining in the islets and the acinar cells, similar to neutral AA transporter (ASCT2) or b0,+-type AA transporter 1(BAT1). Excitatory AA transporter (EAAC) was identified only in the acinar cells. Glutamate was exclusively found in the acinar cells. We revealed the novel dynamics of glutamate in the rat PJ. The glutamate secretion into the PJ was augmented by plasma glutamine, indicating the de novo metabolisms of glutamate, together with the local expression of the related transporters.
D Fukushima; H Doi; K Fukushima; K Katsura; N Ogawa; S Sekiguchi; K Fujimori; A Sato; S Satomi; K Ishida; K Fukushima
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of physiology and pharmacology : an official journal of the Polish Physiological Society     Volume:  61     ISSN:  1899-1505     ISO Abbreviation:  J. Physiol. Pharmacol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-08     Completed Date:  2010-10-21     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  9114501     Medline TA:  J Physiol Pharmacol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  265-71     Citation Subset:  IM    
Advanced Surgical Science and Technology Division, Department of Surgery, Graduate School of Medicine, University of Tohoku, Sendai, Japan.
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MeSH Terms
Amino Acid Transport System X-AG / metabolism*
Amino Acid Transport System y+ / metabolism
Amino Acid Transport Systems / metabolism*
Dipeptides / administration & dosage,  pharmacology
Glutamic Acid / metabolism*
Glutamine / blood*
Islets of Langerhans / metabolism
Large Neutral Amino Acid-Transporter 1 / metabolism
Pancreas / metabolism*
Pancreatic Juice / metabolism
Rats, Sprague-Dawley
Reg. No./Substance:
0/Amino Acid Transport System X-AG; 0/Amino Acid Transport System y+; 0/Amino Acid Transport Systems; 0/Dipeptides; 0/Large Neutral Amino Acid-Transporter 1; 0/xCT protein, rat; 56-85-9/Glutamine; 56-86-0/Glutamic Acid; U5JDO2770Z/alanylglutamine

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