Document Detail

A Glut4-vesicle marker protein, insulin-responsive aminopeptidase, is localized in a novel vesicular compartment in PC12 cells.
MedLine Citation:
PMID:  11489215     Owner:  NLM     Status:  MEDLINE    
Glut4-containing vesicles represent a regulated recycling compartment in insulin-sensitive fat and skeletal muscle cells, the nature and origin of which are not fully understood. In addition to Glut4 itself, these vesicles compartmentalize a number of proteins, at least one of which, insulin-responsive aminopeptidase, or IRAP, is completely colocalized with Glut4 in insulin-sensitive tissues. However, unlike Glut4, IRAP is expressed in a variety of other tissues and cell lines. Here, we explored the intracellular localization of IRAP in the rat pheochromocytoma cell line PC12. We found that this protein is present in a distinct population of slowly recycling light vesicles. By gradient centrifugations, immunoadsorption and double immunofluorescent staining, these vesicles are different from transferrin-containing endosomes, small synaptic vesicles and secretory granules and may thus represent a novel compartment in PC12 cells. Glut4-GFP chimera transiently expressed in PC12 cells is targeted to IRAP-containing vesicles indicating that cotargeting of Glut4 and IRAP is not specific for adipocytes and myocytes, but is faithful in a foreign cell type. We suggest that PC12 cells may possess a novel type of a vesicular carrier that may represent the homolog of Glut4-vesicles.
G Thoidis; K V Kandror
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Traffic (Copenhagen, Denmark)     Volume:  2     ISSN:  1398-9219     ISO Abbreviation:  Traffic     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-07     Completed Date:  2001-11-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100939340     Medline TA:  Traffic     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  577-87     Citation Subset:  IM    
Boston University School of Medicine, Boston, MA 02118, USA.
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MeSH Terms
Centrifugation, Density Gradient
Electrophoresis, Polyacrylamide Gel
Glucose Transporter Type 4
Insulin / metabolism*
Microscopy, Confocal
Microscopy, Fluorescence
Monosaccharide Transport Proteins / biosynthesis*
Muscle Proteins*
PC12 Cells
Subcellular Fractions / metabolism
Time Factors
Grant Support
Reg. No./Substance:
0/Glucose Transporter Type 4; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/Slc2a4 protein, rat; 11061-68-0/Insulin

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