| Glucuronidation of labetalol at the two hydroxy positions by bovine liver microsomes. Isolation, purification, and structure elucidation of the glucuronides of labetalol. | |
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MedLine Citation:
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PMID: 1673399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glucuronidation is known to be a major metabolic pathway for labetalol. As the drug contains a phenolic and an alcoholic hydroxy group, in principle two regio isomeric glucuronides can be formed. By incubating the substrate labetalol with bovine liver microsomes, in the presence of the co-substrate UDP-glucuronic acid, both hydroxy positions were glucuronidated. The different glucuronides were isolated from the incubation mixture using Bond-Elut extraction cartridges and separated by means of high performance liquid chromatography. The formation of glucuronides was confirmed by performing reference incubations using radiolabeled UDP-glucuronic acid. Structure elucidation of the various glucuronides was done by nuclear magnetic resonance, ultraviolet spectroscopy, and mass spectrometry. |
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Authors:
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N R Niemeijer; T K Gerding; R A De Zeeuw |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Drug metabolism and disposition: the biological fate of chemicals Volume: 19 ISSN: 0090-9556 ISO Abbreviation: Drug Metab. Dispos. Publication Date: 1991 Jan-Feb |
Date Detail:
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Created Date: 1991-05-30 Completed Date: 1991-05-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421550 Medline TA: Drug Metab Dispos Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 20-3 Citation Subset: IM |
Affiliation:
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Laboratory Diephuis, Groningen, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Chromatography, High Pressure Liquid Glucuronates / isolation & purification, metabolism Labetalol / metabolism* Magnetic Resonance Spectroscopy Mass Spectrometry Microsomes, Liver / metabolism* Spectrophotometry, Ultraviolet Uridine Diphosphate Glucuronic Acid / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glucuronates; 2616-64-0/Uridine Diphosphate Glucuronic Acid; 36894-69-6/Labetalol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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