Document Detail

Glucosyltransferase mediates adhesion of Streptococcus gordonii to human endothelial cells in vitro.
MedLine Citation:
PMID:  8188339     Owner:  NLM     Status:  MEDLINE    
Human umbilical vein endothelial cells (HUVEC) were used as an experimental host model to investigate the mechanism(s) of streptococcal adhesion in infective endocarditis. Adhesion activity of Streptococcus gordonii was maximal during the logarithmic phase of growth and was greatly reduced or eliminated by pretreatment of bacteria with heat, formaldehyde, or trypsin. At saturating numbers of streptococci, an average of 81 bacteria were bound per HUVEC. Streptococcal adhesion was inhibited by low-molecular-weight dextran and heparin but not by sucrose, fibronectin, or laminin. Adhesion was also prevented by pretreatment of HUVEC with proteins dissociated from the surface of S. gordonii with 10 mM EDTA or isolated from spent culture medium. Western blot (immunoblot) assays detected a single adhesion protein of 153 kDa (AP153) on HUVEC after incubation with unfractionated extracts of streptococci. The adhesin exhibited glucosyltransferase (GTF) activity when incubated with sucrose and Triton X-100 after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The AP153 was purified by affinity chromatography on dextran beads and show to have binding activity for HUVEC, GTF activity, an amino acid composition similar to that reported for GTF of S. gordonii, and the ability to inhibit S. gordonii adhesion. Incubation of the streptococci with antibodies to the adhesin inhibited bacterial attachment to HUVEC monolayers. These results indicate that surface-localized GTF mediates adhesion of S. gordonii to HUVEC in vitro and may serve as a mechanism for colonization of the endocardium in infective endocarditis.
A M Vacca-Smith; C A Jones; M J Levine; M W Stinson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  62     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-06-23     Completed Date:  1994-06-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2187-94     Citation Subset:  IM    
Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo 14214-3005.
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MeSH Terms
Adhesins, Bacterial*
Bacterial Adhesion*
Bacterial Proteins / analysis,  isolation & purification,  physiology*
Cells, Cultured
Endothelium, Vascular / cytology,  microbiology*
Glucosyltransferases / physiology*
Streptococcus / pathogenicity*
Grant Support
Reg. No./Substance:
0/Adhesins, Bacterial; 0/Bacterial Proteins; EC 2.4.1.-/Glucosyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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