Document Detail


Glucose uptake by C6 glioma cells is mediated by G(q alpha).
MedLine Citation:
PMID:  9592059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of G proteins in glucose uptake was investigated using C6 glioma cells. Carbachol (an agonist acting via G protein coupled receptors) and 5'-guanylylimidodiphosphate (Gpp(NH)p; a nonhydrolysable guanine nucleotide analog which bypasses the receptors and directly activates G proteins) stimulated [3H]2-deoxy-D-glucose (2DG) uptake by C6 cells, suggesting that hexose uptake is a G protein-mediated process. To identify the G protein involved in glucose uptake by C6 cells, the effect of carbachol on 2DG uptake was examined in the presence of pertussis toxin. Pertussis toxin treatment did not alter the ability of C6 cells to respond to carbachol, ruling out the involvement of G(i alpha) in 2DG uptake. C6 cells were transfected with G(q alpha) or GLUT1 cDNA for 48 h, exposed to 1 mM carbachol for 2 h, and processed for 2DG uptake. Carbachol stimulated 2DG uptake in both G(q alpha) and GLUT1-transfected cells. Gpp(NH)p, also stimulated 2DG uptake in G(q alpha) and GLUT1-transfected cells. These results suggest that muscarinic receptor coupling to G(q alpha) regulates hexose uptake in C6 cells.
Authors:
S P Singh; Y Gao; S P Kunapuli; R Ravindra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroreport     Volume:  9     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-07-08     Completed Date:  1998-07-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  115-9     Citation Subset:  IM    
Affiliation:
Endocrine-Metabolic Division, Veterans Affairs Medical Center and Finch University of Health Sciences/The Chicago Medical School, North Chicago, IL 60064, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbachol / pharmacology
Deoxyglucose / metabolism
GTP-Binding Proteins / physiology*
Glioma / metabolism*
Glucose / metabolism*
Guanylyl Imidodiphosphate / metabolism
Pertussis Toxin
Rats
Stimulation, Chemical
Tumor Cells, Cultured
Virulence Factors, Bordetella / pharmacology
Grant Support
ID/Acronym/Agency:
R01AA06639/AA/NIAAA NIH HHS
Chemical
Reg. No./Substance:
0/Virulence Factors, Bordetella; 154-17-6/Deoxyglucose; 34273-04-6/Guanylyl Imidodiphosphate; 50-99-7/Glucose; 51-83-2/Carbachol; EC 2.4.2.31/Pertussis Toxin; EC 3.6.1.-/GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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