Document Detail


Glucose transporter protein 1 expression in mucoepidermoid carcinoma of salivary gland: correlation with grade of malignancy.
MedLine Citation:
PMID:  20113375     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mucoepidermoid carcinoma (MEC), the most common primary salivary malignancy, shows great variability in clinical behaviour, thus demanding investigation to identify of prognostic markers. Since Warburg's studies, unrestricted cell growth during tumorigenesis has been linked to altered metabolism, implying hypoxic stimulation of glycolysis and diminished contribution of mitochondrial oxidative phosphorylation to cellular ATP supply. Hypothesizing that the study of MEC metabolic status could lead to the discovery of prognostic markers, we investigated by immunohistochemistry the expression of glucose transporter 1 (Glut-1), mitochondrial antigen and peroxiredoxin I (Prx I) in samples of MEC from different histological grades. Our results showed that mitochondrial antigen and Prx I were expressed in the majority of the MEC cases independent of the histological grade. In contrast Glut-1 expression increased significantly as the tumours became more aggressive. These results suggested that oxidative phosphorylation may contribute to ATP supply in all stages of MEC progression, and that the relative contribution of glycolysis over mitochondria for cellular ATP supply increases during MEC progression, favouring growth under low oxygen concentration. In addition, the observed high Prx I protein levels could provide protection to tumour cells against reactive oxygen species generated as a consequence of mitochondrial function and hypoxia-reoxygenation cycling. Altogether our findings suggest that upregulation of Glut-1 and Prx I constitute successful adaptive strategies of MEC cells conferring a growth advantage over normal salivary gland cells in the unstable oxygenation tumour environment.
Authors:
Ana P D Demasi; Ana F Costa; Albina Altemani; Cristiane Furuse; Ney S Araújo; Vera C Araújo
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Publication Detail:
Type:  Journal Article     Date:  2010-01-21
Journal Detail:
Title:  International journal of experimental pathology     Volume:  91     ISSN:  1365-2613     ISO Abbreviation:  Int J Exp Pathol     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-13     Completed Date:  2010-04-30     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  9014042     Medline TA:  Int J Exp Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  107-13     Citation Subset:  IM    
Affiliation:
Department of Oral Pathology, São Leopoldo Mandic Research Center, Campinas, Brazil. demasiap@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Autoantigens / metabolism
Carcinoma, Mucoepidermoid / immunology,  metabolism*,  pathology
Female
Glucose Transporter Type 1 / metabolism*
Humans
Immunohistochemistry
Male
Middle Aged
Mitochondria / immunology
Peroxiredoxins / metabolism
Salivary Gland Neoplasms / immunology,  metabolism*,  pathology
Tumor Markers, Biological / metabolism
Young Adult
Chemical
Reg. No./Substance:
0/Autoantigens; 0/Glucose Transporter Type 1; 0/Tumor Markers, Biological; EC 1.11.1.15/Peroxiredoxins
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