Document Detail

Glucose sensor using a phospholipid polymer-based enzyme immobilization method.
MedLine Citation:
PMID:  18193406     Owner:  NLM     Status:  MEDLINE    
An electroenzymatic glucose sensor based on a simple enzyme immobilization technique was constructed and tested. The glucose sensor measures glucose concentrations as changes of oxygen concentrations induced by enzymatic reactions. The immobilizing procedure was developed with the purpose of producing wearable biosensors for clinical use. Two types of biocompatible polymers, 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with dodecyl methacrylate (PMD) and MPC copolymerized with 2-ethylhexyl methacrylate, were compared as a sensitive membrane of biosensors. The PMD enzyme membrane had a better response time. Linearity, reproducibility, effect of the concentrations of immobilized enzyme and drifts of sensor characteristics in long-term tests were also investigated. The linear characteristics were confirmed with glucose concentration from 0.01 to 2.00 mmol/l, with a coefficient of determination of 0.9999. The average output current for 1 mmol/l and the standard deviation were 0.992 and 0.0283 muA. Significant changes in the sensor's characteristics were not observed for 2 weeks when it was kept in a refrigerator at 4 degrees C. Because of the simple procedure, the enzyme immobilization method is not only useful for wearable devices but also other devices such as micro total analysis systems.
Hiroyuki Kudo; Tamon Yagi; Ming Xing Chu; Hirokazu Saito; Nobuyuki Morimoto; Yasuhiko Iwasaki; Kazunari Akiyoshi; Kohji Mitsubayashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-13
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  391     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-23     Completed Date:  2008-06-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1269-74     Citation Subset:  IM    
Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan.
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MeSH Terms
Biosensing Techniques / methods*
Enzymes, Immobilized / chemistry,  metabolism
Glucose / analysis*,  metabolism*
Glucose Oxidase / chemistry*,  metabolism*
Methacrylates / chemistry
Molecular Structure
Phospholipids / chemistry*
Phosphorylcholine / analogs & derivatives,  chemistry
Polymers / chemistry*
Reproducibility of Results
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Methacrylates; 0/Phospholipids; 0/Polymers; 0/dodecyl methacrylate; 107-73-3/Phosphorylcholine; 50-99-7/Glucose; 67881-98-5/2-methacryloyloxyethyl phosphorylcholine; EC Oxidase

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