Document Detail


Glucose oversupply increases Delta9-desaturase expression and its metabolites in rat skeletal muscle.
MedLine Citation:
PMID:  12627319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM/HYPOTHESIS: Previous studies have shown that prolonged glucose infusion causes insulin resistance and triglyceride accumulation in rat skeletal muscle. In this study, we investigated a possible relationship between insulin resistance and the composition of different accumulated lipid fractions in rat skeletal muscle. METHODS: Continuous glucose infusion was carried out in rats for 7 days. Lipids were extracted from skeletal muscle, separated by thin layer chromatography and fatty acid composition of phospholipids, triglycerides, diglycerides, free fatty acids and cholesterol esters fractions was analysed by gas chromatography. Delta9-Desaturase mRNA was measured by real time polymerase chain reaction. The enzyme activity was measured in the microsomal fractions. RESULTS: Prolonged glucose infusion (5 days) increased the relative content of palmitoleic acid (16:1 N7) several-fold (2.3- to 5.8-fold) in four out of five lipid fractions and enhanced oleic acid (18:1 N9) two-fold in three lipid fractions suggesting increased Delta9-desaturase activity while the content of several polyunsaturated fatty acids was reduced. In parallel, Delta9-Desaturase mRNA contents and enzyme activities in skeletal muscle were increased 10-fold, 75-fold, 2.6-fold and 7.7-fold after 2 and 5 days of glucose infusion, respectively. CONCLUSION/INTERPRETATION: Our results suggest that long-term glucose oversupply induces a rapid increase in Delta9-desaturase expression and enzyme activity in skeletal muscle which leads to fast and specific changes in fatty acid metabolism possibly contributing to the insulin resistance in this animal model.
Authors:
B Houdali; H G Wahl; M Kresi; V Nguyen; M Haap; F Machicao; H P T Ammon; W Renn; E D Schleicher; H-U Häring
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-01-31
Journal Detail:
Title:  Diabetologia     Volume:  46     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-03-10     Completed Date:  2003-12-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  203-12     Citation Subset:  IM    
Affiliation:
Department of Endocrinology, Metabolism and Pathobiochemistry, Eberhard-Karls-University Tübingen, Otfried-Mueller-Str. 10, 72076 Tübingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Diglycerides / metabolism
Dose-Response Relationship, Drug
Fatty Acids / metabolism
Fatty Acids, Nonesterified / metabolism
Female
Glucose / administration & dosage*
Muscle, Skeletal / enzymology*,  metabolism
Phospholipids / metabolism
RNA, Messenger / metabolism
Rats
Rats, Wistar
Stearoyl-CoA Desaturase / genetics,  metabolism*
Triglycerides / metabolism
Chemical
Reg. No./Substance:
0/Diglycerides; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Phospholipids; 0/RNA, Messenger; 0/Triglycerides; 50-99-7/Glucose; EC 1.14.19.1/Stearoyl-CoA Desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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