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Glucose metabolism in mouse embryos exposed to metformin in vitro.
MedLine Citation:
PMID:  20654465     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Metformin is an oral hypoglycaemic agent used to treat patients with non-insulin-dependent diabetes mellitus, but its effect on embryonic tissues has not been well studied. Early-somite mouse embryos were exposed in whole embryo culture to metformin (0-2000mug/ml) and assayed for glucose uptake and glycolysis at 6, 12 and 24 hours. Embryos exposed to metformin for 6 hours were also evaluated for glucose uptake in the presence of 0 or 100 mum cytochalasin B. Glucose uptake was increased in embryos exposed to metformin at 2000mug/ml for 6 hours and 1000mug/ml or more for 12 hours. Glycolysis was increased in embryos exposed to metformin at 2000mug/ml for 6 or 24 hours and 1000mug/ml or more for 12 hours, producing lactate concentrations up to six times higher than controls. Glut-1 was increased in embryos exposed to 1000mug/ml or more for 6 hours, and metformin-stimulated glucose uptake was significantly decreased by cytochalasin B. Thus, glucose uptake and glycolytic metabolism are increased in early-somite mouse embryos in response to high concentrations of metformin in vitro, and the mechanism of increased glucose uptake appears to involve a cytochalasin B-sensitive glucose transporter.
Authors:
I W Smoak
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  13     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  1999 Feb 
Date Detail:
Created Date:  2010-07-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  -    
Affiliation:
Department of Anatomy, Physiological Sciences and Radiology, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough St, Raleigh, NC 27606, USA.
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