Document Detail


Glucose metabolism in mammalian cell culture: new insights for tweaking vintage pathways.
MedLine Citation:
PMID:  20691487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultured mammalian cells are major vehicles for producing therapeutic proteins, and energy metabolism in those cells profoundly affects process productivity. The characteristic high glucose consumption and lactate production of industrial cell lines as well as their adverse effects on productivity have been the target of both cell line and process improvement for several decades. Recent research advances have shed new light on regulation of glucose metabolism and its links to cell proliferation. This review highlights our current understanding in this area of crucial importance in bioprocessing and further discusses strategies for harnessing new findings toward process enhancement through the manipulation of cellular energy metabolism.
Authors:
Bhanu Chandra Mulukutla; Salmaan Khan; Alex Lange; Wei-Shou Hu
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Publication Detail:
Type:  Journal Article; Review     Date:  2010-08-05
Journal Detail:
Title:  Trends in biotechnology     Volume:  28     ISSN:  1879-3096     ISO Abbreviation:  Trends Biotechnol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8310903     Medline TA:  Trends Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  476-84     Citation Subset:  IM    
Copyright Information:
Copyright 2010. Published by Elsevier Ltd.
Affiliation:
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods*
Glucose / metabolism*
Humans
Metabolic Networks and Pathways
Signal Transduction
Systems Biology / methods*
Chemical
Reg. No./Substance:
50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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