Document Detail


Glucose metabolism in human adipose tissue studied by 13C-glucose and microdialysis.
MedLine Citation:
PMID:  17365995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Microdialysis can be used to monitor carbohydrate metabolism and lipolysis in adipose tissue. This method, however, does not discriminate between local metabolite production and delivery from other tissues. Our aim was to study glucose metabolism by direct delivery of 13C-labelled glucose into adipose tissue by microdialysis. MATERIAL AND METHODS: Seven healthy adults were studied after an overnight fast. In three of them the effect of physical activity on glucose metabolism was tested. Microdialysis catheters were introduced into abdominal adipose tissue and 25 mM 13C-labelled glucose was added to the perfusion fluid. An extraction procedure for separating lactic acid from glucose and glycerol in the microdialysate samples was developed. After derivatization, the 13C enrichment of the compounds was analysed by gas chromatography-mass spectrometry. RESULTS: 13C-labelled lactate was detected in the first 15-min eluate fraction following that in which 13C-glucose had reached the microdialysis probe. In the different subjects, 22-35 % of adipose tissue lactate was produced locally. During exercise there was an increase in the lactate concentration and a decrease in 13C enrichment of lactate. Although lactate production in the adipose tissue increased during exercise, most adipose tissue lactate resulted from inflow. The administered 13C-labelled glucose also rapidly converted to 13C-glycerol. The 13C enrichment of glycerol was lower than that of lactate. During exercise the 13C enrichment of glycerol increased, indicating that newly synthesized depot fat was preferentially hydrolysed during physical activity. CONCLUSIONS: Metabolism of glucose to lactate and glycerol in subcutaneous adipose tissue is a rapid process that can be monitored in vivo by administration of stable isotope labelled glucose into the microdialysis probe. In adults at rest about one-fourth of adipose tissue lactate is produced locally.
Authors:
J Gustafsson; J Eriksson; C Marcus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation     Volume:  67     ISSN:  0036-5513     ISO Abbreviation:  Scand. J. Clin. Lab. Invest.     Publication Date:  2007  
Date Detail:
Created Date:  2007-03-16     Completed Date:  2007-05-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404375     Medline TA:  Scand J Clin Lab Invest     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  155-64     Citation Subset:  IM    
Affiliation:
Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden. Jan.Gustafsson@kbh.uu.se
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / metabolism*
Adult
Carbon Isotopes / diagnostic use
Exercise / physiology
Exercise Test
Female
Gas Chromatography-Mass Spectrometry
Glucose / metabolism*
Glycerol / metabolism
Humans
Lactic Acid / metabolism
Male
Microdialysis*
Subcutaneous Tissue / metabolism*
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 50-21-5/Lactic Acid; 50-99-7/Glucose; 56-81-5/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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