| Glucose-mediated spatial interactions of voltage dependent calcium channels and calcium sensing receptor in insulin producing β-cells. | |
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MedLine Citation:
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PMID: 21146545 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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AIMS: The voltage dependent calcium channel (VDCC) e.g., L-type VDCC plays critical roles in the spatio-temporal regulation of intracellular calcium concentration ([Ca(2+)](i)) and insulin secretion by β-cell. This study describes the involvement of 2.5 to 15mM glucose-induced spatial interactions between a calcium sensing receptor (CaR) and L-type VDCC in controlling Ca(2+) channel activity and insulin secretion in β-cells in association with the nuclear translocation of a transcription factor nuclear factor kappa B (NF-κB). MAIN METHODS: The insulin producing β-cells were exposed to 2.5, 5, 7.5, 10, and 15mM glucose for 24h at 37°C. The confocal fluorescence imaging data was obtained by using antibodies against CaR and L-type VDCC. The nuclear translocation of NF-κB was measured by confocal fluorescence imaging using antibody against NF-κB. The insulin release was determined by enzyme-linked immunosorbent assay (ELISA). KEY FINDINGS: The confocal imaging data showed 6 to 12-fold enhancement in the colocalization correlation coefficient between CaR and VDCC in β-cells exposed to glucose thereby indicating increased membrane delimited spatial interactions between these two membrane proteins. The confocal fluorescence imaging data showed that addition of glucose to β-cells led to 1.8 to 2.7-fold increase in the nuclear translocation of NF-κB. The insulin ELISA data showed a significant increase in the 1st phase of glucose-induced insulin secretion in β-cells exposed to increasing concentrations of glucose. SIGNIFICANCE: The results described in the present study further strengthen that VDCC and CaR can interact spatially to allow control over calcium channel activity and therefore glucose-induced insulin secretion by β-cells. |
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Authors:
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Jai Parkash |
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Publication Detail:
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Type: Journal Article Date: 2010-12-10 |
Journal Detail:
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Title: Life sciences Volume: 88 ISSN: 1879-0631 ISO Abbreviation: Life Sci. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 257-64 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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