| Glucose-induced islet hyperemia is mediated by nitric oxide. | |
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MedLine Citation:
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PMID: 8554133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To determine whether hyperglycemia affects pancreatic islet microcirculation in vivo and whether nitric oxide is a mediator. METHODS: Islet blood flow was measured before and after infusion of glucose during in vivo microscopy of mouse pancreatic islet. The pancreas of male BALB/c mice was exteriorized and viewed under the microscope utilizing monochromatic transmitted light. The carotid artery and tail vein were cannulated and systemic blood pressure was monitored continuously. Under fluorescent light, a 0.02 mL bolus of 2% fluorescein isothyocyanate (FITC-albumin) was injected intra-arterially and the first pulse of FITC-albumin through an islet capillary was videorecorded. Following equilibration, either glucose or normal saline 300 mg/g of body weight was given intravenously. Five minutes later, a second bolus was given and the second pulse was videorecorded. The study was repeated in the presence of N omega-nitro-L-arginine methyl ester (L-NAME). The FITC-albumin bolus mean transit time (TT) and observed cross time (OCT) through the islet were calculated using slow-motion video analysis of the recorded images. RESULTS: Infusion of glucose resulted in a significant increase in islet blood flow with no change in systemic blood pressure: baseline TT was 20 +/- 1.3 pixel/0.03 sec and baseline OCT was 0.6 +/- 0.04 seconds; during hyperglycemia, TT was 16.1 +/- 1 pixel/0.03 sec, and OCT was 0.48 +/- 0.03 seconds (n = 11, P < 0.05 versus basal via paired t-test). Continuous infusion of L-NAME negated the effect of hyperglycemia on islet blood flow: baseline TT was 20 +/- 1.8 pixel/0.03 sec and OCT was and 0.6 +/- 0.05 seconds; during hyperglycemia, TT was 20 +/- 1.1 pixel/0.03 sec and OCT was 0.6 +/- 0.33 seconds (n = 10; P < 0.05 versus glucose via unpaired t-test). |
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Authors:
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S Moldovan; E Livingston; R S Zhang; R Kleinman; P Guth; F C Brunicardi |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of surgery Volume: 171 ISSN: 0002-9610 ISO Abbreviation: Am. J. Surg. Publication Date: 1996 Jan |
Date Detail:
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Created Date: 1996-02-21 Completed Date: 1996-02-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370473 Medline TA: Am J Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 16-20 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, Veterans Affairs Medical Center-West Los Angeles, California, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / analogs & derivatives, pharmacology Blood Pressure Glucose / administration & dosage Hyperglycemia / physiopathology* Islets of Langerhans / blood supply* Male Mice Mice, Inbred BALB C Microcirculation NG-Nitroarginine Methyl Ester Nitric Oxide / physiology* Nitric Oxide Synthase / antagonists & inhibitors |
| Grant Support | |
ID/Acronym/Agency:
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1R29DK 46441-01/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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10102-43-9/Nitric Oxide; 50-99-7/Glucose; 50903-99-6/NG-Nitroarginine Methyl Ester; 74-79-3/Arginine; EC 1.14.13.39/Nitric Oxide Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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