| Glucose increases activity and Ca2+ in insulin-producing cells of adult Drosophila. | |
| | |
MedLine Citation:
|
PMID: 20890228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We sought to understand the mechanisms underlying glucose sensing in Drosophila melanogaster. We found that insulin-producing cells (IPCs) of adult Drosophila respond to glucose and glibenclamide with a burst-like pattern of activity. Under controlled conditions IPCs have a resting membrane potential of -62+/-4 mV. In response to glucose or glibenclamide, IPCs generate action potentials at a threshold of -36+/-1.4 mV with an amplitude of 46+/-4 mV and width of 9.3+/-1.8 ms. Real-time Ca imaging confirms that IPCs respond to glucose and glibenclamide with increased intracellular Ca. These results provide the first detailed characterization of electrical properties of IPCs of adult Drosophila and suggest that these cells sense glucose by a mechanism similar to mammalian pancreatic β cells. |
| | |
Authors:
|
Orsolya Kréneisz; Xinnian Chen; Yih-Woei C Fridell; Daniel K Mulkey |
Related Documents
:
|
10714358 - Insulin resistance and type 2 diabetes mellitus: its relationship with the beta 3-adren... 10318828 - Chronic hyperglycemia triggers loss of pancreatic beta cell differentiation in an anima... 11795838 - Albert renold memorial lecture: molecular background of nutritionally induced insulin r... 2045718 - Effects of glycemic control on red cell deformability determined by using the cell tran... 9571348 - An electronic case manager for diabetes control. 22498478 - Suppression of autophagy is protective in high glucose-induced cardiomyocyte injury. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Neuroreport Volume: 21 ISSN: 1473-558X ISO Abbreviation: Neuroreport Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-03 Completed Date: 2011-09-13 Revised Date: 2011-12-13 |
Medline Journal Info:
|
Nlm Unique ID: 9100935 Medline TA: Neuroreport Country: England |
Other Details:
|
Languages: eng Pagination: 1116-20 Citation Subset: IM |
Affiliation:
|
Departments of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Calcium / physiology* Calcium Signaling / drug effects, physiology Drosophila melanogaster / cytology*, metabolism*, physiology Glucose / metabolism*, pharmacology Glyburide / pharmacology Homeostasis / drug effects, physiology Hypoglycemic Agents / pharmacology Insulin / metabolism, secretion* Insulin-Secreting Cells / cytology, drug effects, physiology* KATP Channels / drug effects, physiology* Membrane Potentials / drug effects, physiology Models, Animal |
| Grant Support | |
ID/Acronym/Agency:
|
R21 AG031086-03/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Hypoglycemic Agents; 0/Insulin; 0/KATP Channels; 10238-21-8/Glyburide; 50-99-7/Glucose; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: ?7 and ?2 nicotinic receptors control monoamine-mediated locomotor response.
Next Document: Hippocampal neurofibrillary tangle changes and aggressive behaviour in dementia.