Document Detail


Glucose and ethylene signalling pathways converge to regulate trans-differentiation of epidermal transfer cells in Vicia narbonensis cotyledons.
MedLine Citation:
PMID:  21848654     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Transfer cells (TCs) are specialized transport cells containing invaginated wall ingrowths that generate an amplified plasma membrane surface area with high densities of transporter proteins. They trans-differentiate from differentiated cells at sites where enhanced rates of nutrient transport occur across apo-/symplasmic boundaries. Despite their physiological importance, the signal(s) and signalling cascades responsible for initiating their trans-differentiation are poorly understood. Placed into culture, adaxial epidermal cells of Vicia narbonensis cotyledons were induced to trans-differentiate to a TC morphology. Manipulating their intracellular glucose concentrations, through transgenic knock-down of ADP-glucose pyrophosphorylase expression and/or culture on a high glucose medium, demonstrated that glucose functioned as a negative regulator of wall ingrowth induction. In contrast, glucose exerted no detectable effect on wall ingrowth morphology. Responses of wall ingrowth induction to culturing cotyledons on media containing glucose analogues suggested glucose acts through a hexokinase-dependent signalling pathway. Elevation of an epidermal cell-specific ethylene signal alone, or in combination with glucose analogues, countered the negative effect of glucose on wall ingrowth induction. Glucose modulated the amplitude of ethylene-stimulated wall ingrowth induction through down regulating expression of ethylene biosynthetic genes and an Ethylene Insensitive 3 (EIN3)-Like gene (EIL) encoding a key transcription factor in the ethylene-signalling cascade. A model is presented describing the interaction between glucose and ethylene signalling pathways regulating induction of wall ingrowth formation in adaxial epidermal cells.
Authors:
Felicity A Andriunas; Hui-Ming Zhang; Hans Weber; David W McCurdy; Christina E Offler; John W Patrick
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-17
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  -     ISSN:  1365-313X     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Blackwell Publishing Ltd and the Society for Experimental Biology.
Affiliation:
School of Environmental & Life Sciences, The University of Newcastle, Callaghan, NSW 2308 Australia. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany.
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