| Glucose-dose dependent characteristics of insulin secretion in obese and lean sheep. | |
| | |
MedLine Citation:
|
PMID: 3297649 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We previously reported that obesity in sheep and cattle was associated with basal hyperinsulinemia, insulin resistance, and an exaggerated insulin response to a single dose (350 mg/kg) of glucose. In this study, the glucose-dose dependency of insulin secretion in obese and lean sheep was determined by 1) using jugular venous concentrations of insulin (Exp 1) and 2) arteriovenous differences in insulin concentrations across the pancreas together with plasma flow rates in the portal vein (Exp 2). Sheep were injected with glucose doses of 0 (water), 10, 30, 100, and 350 mg glucose/kg body weight in Exp 1 (six sheep per group) and with a low (20 mg/kg) and high (200 mg/kg) dose of glucose in exp 2 (four sheep per group). In Exp 1, mean (+/- SE) pretreatment plasma concentrations of insulin (22.0 +/- 1.7 vs. 9.4 +/- 0.4 microU/ml) and glucose (56.1 +/- 0.5 vs. 52.4 +/- 0.8 mg/dl) were greater (P less than 0.01) in obese than lean sheep fasted for 12 h. The glucose-induced rises in insulin concentrations above pretreatment levels were always greater (P less than 0.05) in obese than lean sheep regardless of glucose dose. Eadie-Scatchard plot analysis of the hyperbolic relationship between the acute insulin and acute glucose response areas (0 to +10 min) indicated that the maximum (Vmax) early phase insulin response was greater (P less than 0.025) in obese than lean sheep (568 +/- 148 vs. 156 +/- 33 microU ml-1 X min). In Exp 2, pretreatment concentrations of insulin (25.1 +/- 3.4 vs. 5.6 +/- 1.2 microU/ml) and glucose (58.3 +/- 1.8 vs. 45.5 +/- 1.1 mg/dl) in arterial plasma were greater (P less than 0.01) in obese than in lean sheep fasted 18 to 22 h. Similarly, pretreatment pancreatic secretion rates of insulin were greater (P less than 0.01) in obese (17.8 +/- 5.8 mU/min) than in lean (4.9 +/- 1.3 mU/min) sheep. Glucose-induced acute (0 to +10 min) increments in pancreatic secretory rates of insulin also were greater (P less than 0.05) in obese than in lean sheep after the low (215 +/- 73 vs. 11 +/- 15 mU) and high (881 +/- 281 vs. 232 +/- 66 mU) doses of glucose. It was concluded that insulin secretion in response to a range of stimulatory concentrations of glucose was greater in obese than in lean sheep because the obese sheep had greater maximum (i.e. Vmax) acute phases of glucose-induced insulin secretion. |
| | |
Authors:
|
J P McCann; T J Reimers; E N Bergman |
Related Documents
:
|
1112449 - Lack of dietary regulation of jejunal glycolytic enzymes and disaccharidases in obesity... 8776009 - Effects of exercise training on abdominal obesity and related metabolic complications. 15830179 - Hypoadiponectinaemia and high risk of type 2 diabetes are associated with adiponectin-e... 18255009 - Obesity, diabetes, and chronic kidney disease. 6191939 - Procainamide disposition in obesity. 15543219 - Programming of obesity and cardiovascular disease. 21368719 - In vitro inhibitory effects of cyandin-3-rutinoside on pancreatic α-amylase and its co... 17658679 - Insulin regulation of growth hormone receptor gene expression. evidence for a transcrip... 7037829 - Autoimmunity in type i (insulin-dependent) diabetes mellitus in north india. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Endocrinology Volume: 121 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1987 Aug |
Date Detail:
|
Created Date: 1987-08-25 Completed Date: 1987-08-25 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 553-60 Citation Subset: AIM; IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Flow Velocity Blood Glucose / metabolism Dose-Response Relationship, Drug Female Glucose / pharmacology* Insulin / secretion* Kinetics Obesity / physiopathology, veterinary* Portal Vein / physiopathology Sheep Sheep Diseases* |
| Grant Support | |
ID/Acronym/Agency:
|
AM-05976/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Blood Glucose; 11061-68-0/Insulin; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Glucose and amino acid uptake by exercising muscles in vivo: effect of insulin, fiber population, an...
Next Document: Multihormonal control of synthesis and secretion of prostatein in cultured rat ventral prostate.