| Glucose Sensing by Time-Resolved Fluorescence of Sol-Gel Immobilized Glucose Oxidase. | |
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MedLine Citation:
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PMID: 22163807 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A monolithic silica gel matrix with entrapped glucose oxidase (GOD) was constructed as a bioactive element in an optical biosensor for glucose determination. Intrinsic fluorescence of free and immobilised GOD was investigated in the visible range in presence of different glucose concentrations by time-resolved spectroscopy with time-correlated single-photon counting detector. A three-exponential model was used for analysing the fluorescence transients. Fractional intensities and mean lifetime were shown to be sensitive to the enzymatic reaction and were used for obtaining calibration curve for glucose concentration determination. The sensing system proposed achieved high resolution (up to 0.17 mM) glucose determination with a detection range from 0.4 mM to 5 mM. |
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Authors:
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Rosario Esposito; Bartolomeo Della Ventura; Sergio De Nicola; Carlo Altucci; Raffaele Velotta; Damiano Gustavo Mita; Maria Lepore |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-24 |
Journal Detail:
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Title: Sensors (Basel, Switzerland) Volume: 11 ISSN: 1424-8220 ISO Abbreviation: - Publication Date: 2011 |
Date Detail:
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Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101204366 Medline TA: Sensors (Basel) Country: - |
Other Details:
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Languages: ENG Pagination: 3483-3497 Citation Subset: - |
Affiliation:
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CNISM-Dipartimento Scienze Fisiche, Università di Napoli Federico II, Napoli 80126, Italy; E-Mails: altucci@na.infn.it (C.A.); velotta@na.infn.it (R.V.). |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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