| Glucose Oxidase Incorporated Collagen Matrices for Dermal Wound Repair in Diabetic Rat Models: A Biochemical Study. | |
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MedLine Citation:
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PMID: 21363874 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Impaired wound healing in diabetes is a well-documented phenomenon. Emerging data favor the involvement of free radicals in the pathogenesis of diabetic wound healing. We investigated the beneficial role of the sustained release of reactive oxygen species (ROS) in diabetic dermal wound healing. In order to achieve the sustained delivery of ROS in the wound bed, we have incorporated glucose oxidase in the collagen matrix (GOIC), which is applied to the healing diabetic wound. Our in vitro proteolysis studies on incorporated GOIC show increased stability against the proteases in the collagen matrix. In this study, GOIC film and collagen film (CF) are used as dressing material on the wound of streptozotocin-induced diabetic rats. A significant increase in ROS (p < 0.05) was observed in the fibroblast of GOIC group during the inflammation period compared to the CF and control groups. This elevated level up regulated the antioxidant status in the granulation tissue and improved cellular proliferation in the GOIC group. Interestingly, our biochemical parameters nitric oxide, hydroxyproline, uronic acid, protein, and DNA content in the healing wound showed that there is an increase in proliferation of cells in GOIC when compared to the control and CF groups. In addition, evidence from wound contraction and histology reveals faster healing in the GOIC group. Our observations document that GOIC matrices could be effectively used for diabetic wound healing therapy. |
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Authors:
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V Arul; J G Masilamoni; E P Jesudason; P J Jaji; M Inayathullah; D G Dicky John; S Vignesh; R Jayakumar |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-1 |
Journal Detail:
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Title: Journal of biomaterials applications Volume: - ISSN: 1530-8022 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8813912 Medline TA: J Biomater Appl Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Bio-Organic and Neurochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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