| Glucose-dependent blood flow dynamics in murine pancreatic islets in vivo. | |
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MedLine Citation:
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PMID: 20071562 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pancreatic islets are highly vascularized and arranged so that regions containing beta-cells are distinct from those containing other cell types. Although islet blood flow has been studied extensively, little is known about the dynamics of islet blood flow during hypoglycemia or hyperglycemia. To investigate changes in islet blood flow as a function of blood glucose level, we clamped blood glucose sequentially at hyperglycemic ( approximately 300 mg/dl or 16.8 mM) and hypoglycemic ( approximately 50 mg/dl or 2.8 mM) levels while simultaneously imaging intraislet blood flow in mouse models that express green fluorescent protein in the beta-cells or yellow fluorescent protein in the alpha-cells. Using line scanning confocal microscopy, in vivo blood flow was assayed after intravenous injection of fluorescent dextran or sulforhodamine-labeled red blood cells. Regardless of the sequence of hypoglycemia and hyperglycemia, islet blood flow is faster during hyperglycemia, and apparent blood volume is greater during hyperglycemia than during hypoglycemia. However, there is no change in the order of perfusion of different islet endocrine cell types in hypoglycemia compared with hyperglycemia, with the islet core of beta-cells usually perfused first. In contrast to the results in islets, there was no significant difference in flow rate in the exocrine pancreas during hyperglycemia compared with hypoglycemia. These results indicate that glucose differentially regulates blood flow in the pancreatic islet vasculature independently of blood flow in the rest of the pancreas. |
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Authors:
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Lara R Nyman; Eric Ford; Alvin C Powers; David W Piston |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-01-13 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 298 ISSN: 1522-1555 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-22 Completed Date: 2010-04-13 Revised Date: 2011-07-27 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E807-14 Citation Subset: IM |
Affiliation:
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Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / physiology Glucagon / metabolism Glucose / physiology* Glucose Clamp Technique Green Fluorescent Proteins / genetics Hyperglycemia / metabolism, physiopathology Hypoglycemia / metabolism, physiopathology Islets of Langerhans / blood supply* Mice Mice, Transgenic Microscopy, Confocal Regional Blood Flow / physiology |
| Grant Support | |
ID/Acronym/Agency:
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CA-68485/CA/NCI NIH HHS; DK-07563/DK/NIDDK NIH HHS; DK-20593/DK/NIDDK NIH HHS; DK-53434/DK/NIDDK NIH HHS; DK-58404/DK/NIDDK NIH HHS; DK-59637/DK/NIDDK NIH HHS; DK-63439/DK/NIDDK NIH HHS; DK-66636/DK/NIDDK NIH HHS; DK-68764/DK/NIDDK NIH HHS; DK-69603/DK/NIDDK NIH HHS; EY-08126/EY/NEI NIH HHS; HD-15052/HD/NICHD NIH HHS; RR-22620/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 147336-22-9/Green Fluorescent Proteins; 50-99-7/Glucose; 9007-92-5/Glucagon |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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