Document Detail

Glucose-6-phosphatase activity of endoplasmic reticulum and Golgi apparatus in spermatocytes and spermatids of the rat: an electron microscopic cytochemical study.
MedLine Citation:
PMID:  1655137     Owner:  NLM     Status:  MEDLINE    
The glucose-6-phosphatase (G6Pase) activity of cytoplasmic components of spermatocytes and spermatids of the rat was examined by electron microscope cytochemistry using cerium chloride as a capture agent. G6Pase activity, a recognized ER-resident enzyme, was present in all ER cisternae of spermatocytes. In spermatids, while some ER cisternae were G6Pase-reactive, others were negative or only slightly reactive, indicating an unequal distribution of the enzymatic activity throughout the network of ER cisternae in these cells. In spermatocytes, the cis- and trans-elements of the stacks of Golgi saccules were slightly but significantly reactive for G6Pase. In the Golgi apparatus of spermatids, the cis-element, 4 or 5 underlying saccules, as well as one or two thick trans Golgi elements were G6Pase reactive. The G6Pase activity of the various Golgi elements, like that of the ER cisternae was not affected by the pH of the medium and was completely inhibited by Na-vanadate, a known G6Pase inhibitor. Sertoli and Leydig cells, submitted to the same cytochemical conditions, showed complete G6Pase reactivity of their ER; however in Sertoli cells, all Golgi components were consistently negative while in Leydig cells the cis- and trans-elements of the Golgi stacks were slightly reactive, as in spermatocytes. Thus, the G6Pase reactivity of Golgi elements, appeared variable from one cell type to another. The compact juxtanuclear Golgi apparatuses of spermatocytes and spermatids were both associated with numerous G6Pase reactive ER cisternae; some were present at their surface, others crossed their cortices between Golgi stacks and formed elaborate networks in their cores.(ABSTRACT TRUNCATED AT 250 WORDS)
G Thorne-Tjomsland; Y Clermont; X M Tang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  71     ISSN:  0248-4900     ISO Abbreviation:  Biol. Cell     Publication Date:  1991  
Date Detail:
Created Date:  1991-10-31     Completed Date:  1991-10-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  33-41     Citation Subset:  IM    
Department of Anatomy, McGill University, Montreal, Quebec, Canada.
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MeSH Terms
Endoplasmic Reticulum / enzymology*,  ultrastructure
Glucose-6-Phosphatase / antagonists & inhibitors,  metabolism*
Golgi Apparatus / enzymology*,  ultrastructure
Hydrogen-Ion Concentration
Microscopy, Electron
Spermatids / enzymology*,  ultrastructure
Spermatocytes / enzymology*,  ultrastructure
Substrate Specificity
Reg. No./Substance:

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