Document Detail


Glucose-6-phosphate dehydrogenase protects Escherichia coli from tellurite-mediated oxidative stress.
MedLine Citation:
PMID:  21984934     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The tellurium oxyanion tellurite induces oxidative stress in most microorganisms. In Escherichia coli, tellurite exposure results in high levels of oxidized proteins and membrane lipid peroxides, inactivation of oxidation-sensitive enzymes and reduced glutathione content. In this work, we show that tellurite-exposed E. coli exhibits transcriptional activation of the zwf gene, encoding glucose 6-phosphate dehydrogenase (G6PDH), which in turn results in augmented synthesis of reduced nicotinamide adenine dinucleotide phosphate (NADPH). Increased zwf transcription under tellurite stress results mainly from reactive oxygen species (ROS) generation and not from a depletion of cellular glutathione. In addition, the observed increase of G6PDH activity was paralleled by accumulation of glucose-6-phosphate (G6P), suggesting a metabolic flux shift toward the pentose phosphate shunt. Upon zwf overexpression, bacterial cells also show increased levels of antioxidant molecules (NADPH, GSH), better-protected oxidation-sensitive enzymes and decreased amounts of oxidized proteins and membrane lipids. These results suggest that by increasing NADPH content, G6PDH plays an important role in E. coli survival under tellurite stress.
Authors:
Juan M Sandoval; Felipe A Arenas; Claudio C Vásquez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-30
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-10-10     Completed Date:  2012-03-05     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e25573     Citation Subset:  IM    
Affiliation:
Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
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MeSH Terms
Descriptor/Qualifier:
Escherichia coli / drug effects*,  enzymology*,  metabolism
Escherichia coli Proteins / genetics,  metabolism*
Glucose-6-Phosphate / metabolism
Glucosephosphate Dehydrogenase / genetics,  metabolism*
NADP / metabolism
Oxidative Stress / drug effects*
Reactive Oxygen Species / metabolism
Tellurium / pharmacology*
Chemical
Reg. No./Substance:
0/Escherichia coli Proteins; 0/Reactive Oxygen Species; 13494-80-9/Tellurium; 53-59-8/NADP; 56-73-5/Glucose-6-Phosphate; EC 1.1.1.49/Glucosephosphate Dehydrogenase; IVA6SGP6QM/tellurous acid
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