Document Detail

Glucosamine regulates differentiation of a chondrogenic cell line, ATDC5.
MedLine Citation:
PMID:  17329833     Owner:  NLM     Status:  MEDLINE    
Osteoarthritis (OA) is a slowly progressing chronic joint disease. Glucosamine (GlcN) is a saccharide that is widely used to relieve symptoms associated with OA. However, the mechanism of the effects of GlcN on articular cartilage remains unclear. We studied the effects of GlcN and its analogues, including chitin derivatives included in health supplements containing GlcN, on a chondrogenic cell line, ATDC5. We examined the effects of these saccharides on the proliferation and differentiation of ATDC5 cells. Glucosamine analogues, such as N-acetyl glucosamine and chitobiose, did not affect the proliferation or differentiation of ATDC5 cells. While GlcN did not affect the proliferation of ATDC5 cells, it inhibited their differentiation. Next, we examined whether GlcN affects mineralization and glycosaminoglycan (GAG) production by ATDC5 cells. Mineralization was markedly inhibited by addition of GlcN to the cell culture medium. Moreover, GlcN induced the formation of sulfated GAG in ATDC5. We also analyzed the mRNA levels in ATDC5 cells. GlcN reduced the mRNA levels of Smad2, Smad4 and MGP. GlcN might inhibit expression of MGP mRNA and induce the production of chondroitin sulfate in ATDC5 cells. The mechanism by which GlcN inhibits mineralization may be by regulating the expression of mRNA for the Smad2 and Smad4 chondrogenic master genes.
Sachie Nakatani; Hiroshi Mano; Ryanghyok Im; Jun Shimizu; Masahiro Wada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  30     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-01     Completed Date:  2007-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  433-8     Citation Subset:  IM    
Department of Food Functional Science, Graduate School of Pharmacology, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0248, Japan.
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MeSH Terms
Alkaline Phosphatase / antagonists & inhibitors
Calcification, Physiologic / drug effects
Calcium-Binding Proteins / genetics,  metabolism
Cartilage / drug effects,  physiology
Cell Differentiation / drug effects*
Cell Line
Cell Proliferation / drug effects
Chondrocytes / cytology,  drug effects*,  metabolism
Chondrogenesis / drug effects,  genetics
Extracellular Matrix Proteins / genetics,  metabolism
Gene Expression / drug effects
Gene Expression Profiling
Glucosamine / analogs & derivatives,  metabolism,  pharmacology*
Glycosaminoglycans / metabolism
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Smad2 Protein / genetics,  metabolism
Smad4 Protein / genetics,  metabolism
Sulfates / metabolism
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Extracellular Matrix Proteins; 0/Glycosaminoglycans; 0/RNA, Messenger; 0/Smad2 Protein; 0/Smad4 Protein; 0/Sulfates; 0/matrix Gla protein; 3416-24-8/Glucosamine; EC Phosphatase

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